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Francisella sp. W12-1067

ABSTRACT: Francisella sp. W12-1067 Genome sequencing and assembly

PROVIDER: PRJNA214898 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	AWHF01.dat.gz	Other
	AWHF01.fasta.gz	Fasta.gz
	AWHF01.master.dat	Other
	AWHF01.dat.gz	Other

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1 - 4 of 4

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Prochlorococcus sp. W12

Project description:Prochlorococcus sp. W12 Genome sequencing and assembly

| PRJNA169370 | ENA

Genome sequence and phenotypic analysis of a first German Francisella sp. isolate (W12-1067) not belonging to the species Francisella tularensis.

Project description:BackgroundFrancisella isolates from patients suffering from tularemia in Germany are generally strains of the species F. tularensis subsp. holarctica. To our knowledge, no other Francisella species are known for Germany. Recently, a new Francisella species could be isolated from a water reservoir of a cooling tower in Germany.ResultsWe identified a Francisella sp. (isolate W12-1067) whose 16S rDNA is 99% identical to the respective nucleotide sequence of the recently published strain F. guangzhouensis. The overall sequence identity of the fopA, gyrA, rpoA, groEL, sdhA and dnaK genes is only 89%, indicating that strain W12-1067 is not identical to F. guangzhouensis. W12-1067 was isolated from a water reservoir of a cooling tower of a hospital in Germany. The growth optimum of the isolate is approximately 30°C, it can grow in the presence of 4-5% NaCl (halotolerant) and is able to grow without additional cysteine within the medium. The strain was able to replicate within a mouse-derived macrophage-like cell line. The whole genome of the strain was sequenced (~1.7 mbp, 32.2% G + C content) and the draft genome was annotated. Various virulence genes common to the genus Francisella are present, but the Francisella pathogenicity island (FPI) is missing. However, another putative type-VI secretion system is present within the genome of strain W12-1067.ConclusionsIsolate W12-1067 is closely related to the recently described F. guangzhouensis species and it replicates within eukaryotic host cells. Since W12-1067 exhibits a putative new type-VI secretion system and F. tularensis subsp. holarctica was found not to be the sole species in Germany, the new isolate is an interesting species to be analyzed in more detail. Further research is needed to investigate the epidemiology, ecology and pathogenicity of Francisella species present in Germany.

| S-EPMC4230796 | biostudies-literature

Massilia sp. W12

Project description:Massilia sp. W12 Genome sequencing

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Xylaria sp. BCC 1067

Project description:Xylaria sp. BCC 1067 Genome sequencing and assembly

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Xylaria sp. BCC 1067

Project description:Genome sequence of Xylaria sp. BCC 1067

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Francisella sp.

Project description:Francisella sp. Genome sequencing and assembly

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Mycoplasmopsis bovis 1067

Project description:Mycoplasmopsis bovis 1067 Genome sequencing and assembly

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Francisella sp. MA067296

Project description:Francisella sp. MA067296 genome sequencing and assembly

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Legionella pneumophila strain:1067

Project description:Legionella pneumophila strain:1067 Genome sequencing and assembly

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Next Generation Sequencing (NGS) for quantitative transcriptomic analysis of Francisella strains (Francisella novicida, ribD-deficient Francisella novicida , Francisella tularensis)

Project description:To understand differences of gene expression profiles between Francisella strains RNA profiles of Francisella strains were generated by deep sequencing, in triplicate, using NovaSeq6000. qRT–PCR validation was performed using SYBR Green assays. Our study represents the first detailed differential transcriptomic analysis of Francisella strains , with biologic replicates, generated by RNA-seq technology.

2022-05-31 | GSE174793 | GEO

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