Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Evaluation of quantitative miRNA gene expression platforms in the microRNA Quality Control (miRQC) study

ABSTRACT: Evaluation of quantitative miRNA gene expression platforms in the microRNA Quality Control (miRQC) study

PROVIDER: PRJNA214981 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR950876.fastq.gz	Fastqsanger.gz
	SRR950877.fastq.gz	Fastqsanger.gz
	SRR950878.fastq.gz	Fastqsanger.gz
	SRR950879.fastq.gz	Fastqsanger.gz
	SRR950880.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Evaluation of quantitative miRNA gene expression platforms in the microRNA Quality Control (miRQC) study

Project description:MiRNAs are important negative regulators of protein coding gene expression, and have been studied intensively over the last few years. Several measurement platforms, designed to determine their relative RNA abundance levels in biological samples, have been developed. In this study, we systematically compared 12 commercially available miRNA expression platforms by measuring an identical set of 20 standardized positive and negative control samples, including human universal reference RNA, human brain RNA and titrations thereof, human serum samples, and synthetic spikes from miRNA family members with varying homology. We developed novel and robust quality metrics to objectively assess platform performance of very different technologies such as small RNA sequencing, RT-qPCR and (microarray) hybridization. We assessed reproducibility, sensitivity, accuracy, specificity, and concordance of differential expression. The results indicate that each method has its strengths and weaknesses, which helps to guide informed selection of a quantitative miRNA gene expression platform in function of particular study goals. Sequencing of 20 miRQC samples on Illumina Genome Analyzer IIx System

2013-08-31 | E-GEOD-49816 | biostudies-arrayexpress

Evaluation of quantitative miRNA gene expression platforms in the microRNA Quality Control (miRQC) study

2013-08-31 | GSE49816 | GEO

Evaluation of quantitative miRNA gene expression platforms - custom microRNA GeneChip

Project description:MicroRNA microarray expression dataset used to develop novel and robust quality metrics to objectively assess platform performance of very different technologies.

2013-08-09 | GSE49661 | GEO

Evaluation of quantitative miRNA gene expression platforms - custom microRNA GeneChip

Project description:MicroRNA microarray expression dataset used to develop novel and robust quality metrics to objectively assess platform performance of very different technologies. The HsMir v1s520779F custom array is primarly based on the commercially available Affymetrix microRNA microarray version 2.0 (Affymetrix GeneChip miRNA 2.0 Array datasheet P/N EXP00180 Rev 1) with i) microRNA probe sequences updated using miRBase version 17 ii) all human pre-microRNAs removed iii) all non-human sequences removed iv) all viral target sequences removed. In summary, the array contains 1738 mature microRNAs and 2333 other small RNAs (such as small nucleolar RNAs or small cajal body-specific RNAs) probe sequences plus the Affymetrix hybridization and normalization control probesets.

2013-08-09 | E-GEOD-49661 | biostudies-arrayexpress

Evaluation of single-Cell RNA Sequencing Platforms

Project description:Evaluation of single-Cell RNA Sequencing Platforms

| PRJNA597861 | ENA

Performance Evaluation of Commercial miRNA Expression Array Platforms

2010-03-18 | GSE19248 | GEO

Performance Evaluation of Commercial miRNA Expression Array Platforms

Project description:This data is part of a miRNA platform comparison study. We compared the performance characteristics of four commercial miRNA array technologies and found that all platforms performed well in separate measures of performance. The Ambion and Agilent platforms were more accurate, whereas the Illumina and Exiqon platforms were more specific. Furthermore, the data analysis approach had a large impact on the performance, predominantly by improving precision. Performance of four (4) commercially available miRNA platforms was evaluated using 7 placenta samples spiked with synthetic microRNA spikes (in Latin-square design) absent in placenta. Platforms were primarily evaluated for accuracy and specificity.

2010-06-23 | E-GEOD-19248 | biostudies-arrayexpress

Fluidigm96 - Evaluation of single-Cell RNA Sequencing Platforms

Project description:Fluidigm96 - Evaluation of single-Cell RNA Sequencing Platforms

| PRJNA597871 | ENA

Wafergen - Evaluation of single-Cell RNA Sequencing Platforms

Project description:Wafergen - Evaluation of single-Cell RNA Sequencing Platforms

| PRJNA597868 | ENA

SureCell - Evaluation of single-Cell RNA Sequencing Platforms

Project description:SureCell - Evaluation of single-Cell RNA Sequencing Platforms

| PRJNA597869 | ENA