Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mycobacterium tuberculosis variant bovis BCG

ABSTRACT: Experimental selection of long-term intracellular mycobacteria

PROVIDER: PRJNA215740 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2

Project description:Ketogenesis: An Achilles' heel of starvation-selected persistent mycobacteria

| PRJNA278166 | ENA

Dissecting the Mycobacterium bovis BCG response to macrophage infection to identify targets for anti-tuberculosis drug and vaccine discovery

Project description:New strategies are required to reduce the worldwide burden of tuberculosis. Intracellular survival and replication of Mycobacterium tuberculosis after macrophage phagocytosis is a fundamental step in the complex host-pathogen interactions that lead to granuloma formation and disease. Greater understanding of how the bacterium survives and thrives in these environments will inform novel drug and vaccine discovery programmes. Here, we use in-depth RNA sequencing of Mycobacterium bovis BCG from human THP-1 macrophages to describe the mycobacterial adaptations to the intracellular environment. We identify 329 significantly differentially regulated genes, highlighting cholesterol catabolism, methyl-citrate cycle and iron homeostasis as important for mycobacteria inside macrophages. Focused analysis of PE/PPE and cytochrome P450 gene families highlight additional pathways that are upregulated (35 and five respectively) 24h after infection. Comparison of the intracellular transcriptome to gene essentiality and immunogenicity studies identified 15 potential targets that are both required for intracellular survival and induced on infection, and eight genes upregulated that have been demonstrated to be immunogenic in TB patients. Further insight into these new and established targets will support drug and vaccine development efforts.

2022-01-14 | E-MTAB-11107 | biostudies-arrayexpress

Mycobacterium tuberculosis variant bovis BCG

Project description:Mycobacterium tuberculosis variant bovis BCG Genome sequencing and assembly

| PRJNA247465 | ENA

Mycobacterium tuberculosis variant bovis BCG

Project description:Mycobacterium tuberculosis variant bovis BCG Genome sequencing

| PRJNA256163 | ENA

Mycobacterium tuberculosis variant bovis BCG

Project description:Mycobacterium tuberculosis variant bovis BCG Genome sequencing and assembly

| PRJNA575846 | ENA

Mycobacterium tuberculosis variant bovis BCG

Project description:Mycobacterium bovis BCG (Tokyo type I) resistant to an anti-mycobacterial drug

| PRJDB2393 | ENA

Mycobacterium tuberculosis variant bovis BCG

Project description:Transcriptome analysis of the Tap efflux pump mutant in Mycobacterium bovis BCG.

| PRJNA147361 | ENA

Mycobacterium tuberculosis variant bovis BCG

Project description:Complete genome sequencing and assembly of Mycobacterium tuberculosis variant bovis strain BCG SL 222 Sofia (descending from Russian BCG-I strain)

| PRJNA673391 | ENA

Mycobacterium tuberculosis variant bovis BCG

Project description:Gene Expression profiling of M. bovis BCG strain under hypoxia

| PRJNA105573 | ENA

Mycobacterium tuberculosis variant bovis BCG

Project description:Mycobacterium tuberculosis variant bovis BCG Genome sequencing and assembly

| PRJNA1078279 | ENA