Ontology highlight
ABSTRACT:
PROVIDER: PRJNA215857 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In situ synthesis of peptide microarrays - shadow mask design [3]
| PRJNA217964 | ENA
Project description:In situ synthesis of peptide microarrays - shadow mask design [1]
| PRJNA213301 | ENA
Project description:In situ synthesis of peptide microarrays - shadow mask design [2]
| PRJNA215859 | ENA
Project description:A computer program was used to create random amino acid sequences based on and restricted by physical shadow masks which will be used for lithography-based synthesis of peptides. The output from this algorithm was used to create peptides that were synthesized by Sigma Aldrich, and printed onto glass slides. The arrays contained 384 peptides printed in duplicate for each of 4 different mask designs. 52 different monoclonal antibodies were incubated on these microarrays and analyzed for their propensity to bind the peptides created from each mask set. The diversity of binding served as a proxy for the 'randomness' of these peptides, and provided information about how many masks are needed to truly generate random sequence peptides. two replicates of each peptide was printed on 1 Mask peptide microarray. A minimum of Two microarrays were tested for each sample. Image was qualified using in-house metrics for quality assurance.
2014-01-16 | E-GEOD-49217 | biostudies-arrayexpress
Project description:In this paper several computer programs were used to simulate in situ synthesis of peptides using shadow masks and BOC synthesis. The peptides were designed to be random, or pseudo-random, but fulfill requirements of immunosignaturing. This file contains data from actual 330,000 peptide arrays that used the first iteration of the peptide generation algorithm. Monoclonal antibodies were bound to the microarrays and the total number of peptides that distinguished each monoclonal was measured. This provides a baseline against which to compare purely random sequences.
2014-01-16 | GSE50571 | GEO
Project description:In this paper several computer programs were used to simulate in situ synthesis of peptides using shadow masks and BOC synthesis. The peptides were designed to be random, or pseudo-random, but fulfill requirements of immunosignaturing. This file contains data from actual 330,000 peptide arrays that used the first iteration of the peptide generation algorithm. Monoclonal antibodies were bound to the microarrays and the total number of peptides that distinguished each monoclonal was measured. This provides a baseline against which to compare purely random sequences. One replicate of each peptide was printed on 1 330k peptide microarray. One microarray were tested for each sample. Image was qualified using in-house metrics for quality assurance.
2014-01-16 | E-GEOD-50571 | biostudies-arrayexpress
Project description:A computer program was used to create random amino acid sequences based on and restricted by physical shadow masks which will be used for lithography-based synthesis of peptides. The output from this algorithm was used to create peptides that were synthesized by Sigma Aldrich, and printed onto glass slides. The arrays contained 384 peptides printed in duplicate for each of 4 different mask designs. 52 different monoclonal antibodies were incubated on these microarrays and analyzed for their propensity to bind the peptides created from each mask set. The diversity of binding served as a proxy for the 'randomness' of these peptides, and provided information about how many masks are needed to truly generate random sequence peptides.
2014-01-16 | GSE49217 | GEO
Project description:Purpose: Here, we describe a method, Array-seq, to repurpose classical oligonucleotide microarrays for spatial transcriptomics profiling. We demonstrate that Array-seq yields spatial transcriptomes with high detection sensitivity and localization specificity using histological sections from mouse tissues as test systems. Moreover, we show that the large surface area of Array-seq slides enables the generation of spatial transcriptomes at high throughput by profiling multi-organ sections, in three dimensions by processing serial sections from one sample, and across whole human organs using spleen sections. Experimental Methods: To generate Array-seq slides, we first obtain microarrays carrying custom-design probes that contain common sequences flanking unique barcodes at known coordinates. Second, we perform a simple, two-step reaction that produces mRNA capture probes across all spots on the microarray and thereby creates Array-seq slides set for spatial transcriptomics. Following in-situ mRNA capture, reverse transcription, libaray preparation, Array-seq libraries are sequenced using the Illumina sequencing-by-synthesis (SBS) platform.
2024-11-14 | GSE266244 | GEO
Project description:Intervention1: Tracheal intubation with supraglottic Airway devices: Comparison of tracheal intubation with Ambu AuraGain,fastrach Laryngeal Mask and Blockbuster Laryngeal Mask Airway.
Control Intervention1: Ambu AuraGain: Tracheal intubation with Ambu AuraGain
Control Intervention2: Fastrach Laryngeal Mask Airway: Tracheal intubation with Fastrach Laryngeal Mask Airway.
Control Intervention3: Blockbuster Laryngeal Mask Airway: Tracheal intubation with Blockbuster Laryngeal Mask Airway.
Primary outcome(s): First Attempt success rate of tracheal intubation.Timepoint: During intubation
Study Design: Randomized, Parallel Group Trial
Method of generating randomization sequence:Computer generated randomization Method of allocation concealment:Sequentially numbered, sealed, opaque envelopes Blinding and masking:Participant and Outcome Assessor Blinded
| 2605805 | ecrin-mdr-crc
Project description:Biogenic volatile organic compounds sampled from Australian benthic primary producers in situ, using a novel incubation chamber design.
2024-11-24 | MSV000096512 | MassIVE