ABSTRACT: Expression data from striatum of a mouse model of Huntington´s disease (HD) (HdhQ111/Q111) crossed with mGluR5 knockout mice (mGluR5-/-) and their respective controls (HdhQ20/Q20 and mGluR5+/+).
Project description:To try to investigate the mechanism behind the adaptive phenotypes observed in a mice model model of HD crossed with mGluR5 knockout, we analyzed whether mutated huntingtin (Htt) expression in a mGluR5 null background could be altering the expression of genes that might be involved in the pattern of Htt aggregation and HD-related locomotor alterations. In this data set, we include analysis of gene expression in striatum of mice with four different genotypes: HdhQ20/Q20/mGluR5+/+; HdhQ20/Q20/mGluR5-/- ; HdhQ111/Q111/mGluR5+/+ ; HdhQ111/Q111/mGluR5-/-
Project description:To try to investigate the mechanism behind the adaptive phenotypes observed in a mice model model of HD crossed with mGluR5 knockout, we analyzed whether mutated huntingtin (Htt) expression in a mGluR5 null background could be altering the expression of genes that might be involved in the pattern of Htt aggregation and HD-related locomotor alterations. In this data set, we include analysis of gene expression in striatum of mice with four different genotypes: HdhQ20/Q20/mGluR5+/+; HdhQ20/Q20/mGluR5-/- ; HdhQ111/Q111/mGluR5+/+ ; HdhQ111/Q111/mGluR5-/- 12 samples were analyzed. We used Partek Genomics Suite v6.5 (Partek, St. Louis, MO) to determine differences in gene expression levels. Genotype effects were considered significant based of the following criteria: (i) ANOVA p-values< 0.05 and (ii) 1.5 fold increase or decrease.
Project description:Striatum was isolated from 12-month-old Msh3-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Genomic analysis (ATACseq) was performed on 4 genotypes: WT, Msh3-/-, Het (Q140) KI, and Het (Q140) KI X Msh3-/-, 6 replicates per genotype.
Project description:Striatum was isolated from 12-month-old Msh3+/- and Msh3-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (snRNASeq) was performed on 6 genotypes: WT, Q140, Msh3+/-, Q140 X Msh3+/-, Msh3-/-, and Q140 X Msh3-/-, 4 replicates per genotype.
Project description:Striatum was isolated from 6-month-old Ccdc82+/- and Ccdc82-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Ccdc82+/-, Q140 X Ccdc82+/-, Ccdc82-/-, and Q140 X Ccdc82-/-, 7-8 replicates per genotype.
Project description:Striatum was isolated from 4-month-old Mlh1+/- and Mlh1-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Mlh1+/-, Q140 X Mlh1+/-, Mlh1-/-, and Q140I X Mlh1-/-, 7-9 replicates per genotype.
Project description:Striatum was isolated from 6-month-old Pms2+/- and Pms2-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Pms2+/-, Q140 X Pms2+/-, Pms2-/-, and Q140 X Pms2-/-, 6-12 replicates per genotype.
Project description:Striatum was isolated from 6-month-old Msh3+/- and Msh3-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Msh3+/-, Q140 X Msh3+/-, Msh3-/-, and Q140 X Msh3-/-, 7-8 replicates per genotype.
Project description:Cerebral cortex and striatum were isolated from 6-month old Msh6+/- and Msh6-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Q140, Msh6+/-, Q140 X Msh6+/-, Msh6-/-, and Q140 X Msh6-/-, 8 replicates per genotype.
Project description:As part of collaboration between UCLA and CHDI, UCLA is creating knockouts (KOs) of 123 genes, implicated in Huntington’s disease (HD) through various computational modeling efforts. Striatum and cortex were isolated from 6-month-old Mlh1+/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 4 genotypes: WT, Mlh1+/-, Q140, and Q140 X Mlh1+/-, 8 replicates per genotype.
