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Drosophila melanogaster

ABSTRACT: Mining gene expression data for low doses of radiation and pollutants (dioxin, toluene, formaldehyde)

PROVIDER: PRJNA217367 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR958060.fastq.gz	Fastqsanger.gz
	SRR958061.fastq.gz	Fastqsanger.gz
	SRR958062.fastq.gz	Fastqsanger.gz
	SRR958063.fastq.gz	Fastqsanger.gz
	SRR958064.fastq.gz	Fastqsanger.gz

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Mining gene expression data for low doses of radiation and pollutants (dioxin, toluene, formaldehyde)

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Mining gene expression data for low doses of radiation and pollutants (dioxin, toluene, formaldehyde)

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Project description:We investigate the biological effects of radiation using Drosophila Melanogaster as a model organism, focusing on gene expression and lifespan analysis to determine the effect of different radiation doses. Our results support a threshold effect in response to radiation: no effect on lifespan and no permanent effect on gene expression is seen at doses below 10,000 Roentgens. Adult male Drosophila were irradiated 2 days after eclosion, with one of 6 radiation doses: 10; 1,000; 5,000; 10,000; 20,000 Roentgens. Samples were taken at 3 time points (2, 10 and 20 days post-irradiation).

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Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) on Drosophila melanogaster. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes.

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