Tabanus nigrovittatus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA218953 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR976966.fastq.gz | Fastqsanger.gz |
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Project description:Tabanus nipponicus is a hematophagous insect species with high activity in summer, and its salivary gland secretions play a critical role in mediating successful blood-feeding. In this study, pooled salivary gland samples from three T. nipponicus individuals collected during peak summer activity were used for RNA-seq analysis. Total RNA was extracted under sterile and low-temperature conditions prior to sequencing. Paired-end sequencing generated 45,310,280 raw reads, with 88.75% of reads successfully mapped. De novo assembly yielded 35,175 unigenes, which were aligned to the Swiss-Prot database to obtain UniProt IDs. These IDs were mapped to the GO database, and classified into three GO categories; functional annotation revealed enrichment in metabolic, membrane-related biological processes, and binding/catalytic molecular activities, which underpin the synthesis and secretion of anticoagulant saliva components essential for blood-feeding adaptation and microbial stress resistance. Alignment with the KEGG database identified KEGG IDs, with unique KOs following mapping, and subsequent KEGG enrichment analysis was performed. This high-quality transcriptomic dataset represents the first gene expression profile for T. nipponicus, and builds on previous experimental foundations to provide valuable insights into key physiological processes in tabanids, including anticoagulant, blood meal digestion, antioxidant defense, mammalian host immune interaction, and microbial resistance.
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Project description:Tabanus nipponicus is a hematophagous insect species with high activity in summer, and its midguts play a critical role in successful blood-feeding, as they are closely associated with blood meal digestion, nutrient absorption, and physiological regulation during hematophagy. In this study, midgut samples from three independent T. nipponicus individuals collected during the peak of summer activity were used for RNA-seq analysis. Prior to sequencing, total RNA was extracted under sterile and low-temperature conditions to ensure RNA integrity. Paired-end sequencing generated 43,569,311 to 46,711,652 raw reads, which were further processed for de novo assembly, yielding 8,039 unigenes. These unigenes were first aligned to the Nr database to obtain functional annotations, and the annotated unigene IDs were subsequently mapped to the GO database, being classified into three main GO categories (biological process, cellular component, and molecular function). Functional annotation revealed enrichment in metabolic processes, membrane-related biological processes, and binding/catalytic molecular activities—all of which are closely related to the physiological functions of the midgut in T. nipponicus, including the digestion and absorption of blood meals, the transport of nutrients and metabolites, and the regulation of physiological processes required for continuous blood-feeding, thereby underpinning the synthesis and secretion of anticoagulant saliva components essential for blood-feeding adaptation and microbial stress resistance. Additionally, the unigenes were aligned to the KEGG database to obtain corresponding KEGG IDs, which were then mapped to unique KOs followed by KEGG enrichment analysis to explore the key metabolic pathways involved. Future studies will focus on the key physiological processes of T. nipponicus midguts, including anticoagulation, blood meal digestion, antioxidant defense, and microbial resistance.
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