Project description:Myeloid-derived suppressor cells (MDSCs) are one of the main cell populations that negatively regulate immune responses. However, the mechanism underlying the differentiation and expansion of MDSCs remains unclear. Employing a low-density miRNA microarray and a TaqMan probe-based qRT-PCR assay, we investigated the miRNAs expression profiles of MDSC. Compared to freshly isolated BM cells, BM-MDSCs had a significantly altered miRNA profile. There were 45 miRNAs that were upregulated and 17 miRNAs that were downregulated more than 2-fold. Among these miRNAs, miR-155 and miR-21 have the highest upregulation after cytokine induction.
Project description:Myeloid-derived suppressor cells (MDSCs) are one of the main cell populations that negatively regulate immune responses. However, the mechanism underlying the differentiation and expansion of MDSCs remains unclear. Employing a low-density miRNA microarray and a TaqMan probe-based qRT-PCR assay, we investigated the miRNAs expression profiles of MDSC. Compared to freshly isolated BM cells, BM-MDSCs had a significantly altered miRNA profile. There were 45 miRNAs that were upregulated and 17 miRNAs that were downregulated more than 2-fold. Among these miRNAs, miR-155 and miR-21 have the highest upregulation after cytokine induction. BM cells were planted into dishes using RPMI1640 medium supplemented with 2 mM L-glutamine, 10mM HEPES, 20M-BM-5M 2-mercaptoethanol, 150 U/ml streptomycin, 200 U/ml penicillin, 10% FBS and stimulated with combinations of GM-CSF (40ng/ml), and IL-6 (40ng/ml). Cells were cultured at 37M-BM-0C in 5% CO2-humidified atmosphere for 4 days.Total RNA were isolated, low-density miRNA microarray was performed
Project description:This is a ODE-based mathematical model featuring equations describing the dynamics of tumor cells, cytotoxic T cells, natural killer cells, and myeloid-derived suppressor cells (MDSCs) that together describe the tumor-induced immunosuppression caused by MDSCs.
Project description:The aim of the study is to evaluate whether the preoperative level of myeloid-derived suppressor cells is associated with postoperative complications classified by Clavien-Dindo categories. Levels of all MDSC, polymorphonuclear MDSC (PMNMDSC), monocytic MDSC (MMDSC), early-stage MDSC (EMDSC) and monocytic to polymorphonuclear MDSC ratio (M/PMN MDCS) were established and compared in patients with postoperative complications, severe postoperative complications (>= IIIA according to Clavien-Dindo) and severe septic complications.
