Project description:To explore whether Piezo-CAP induced apoptosis and autophagy through inhibiting multiple cancer survival pathways together of human hepatocellular carcinoma cells in vitro and in vivo. RNA-sequence (RNA-seq), Western blot and quantitative real-time PCR (qRT-PCR) were utilized to investigate the mechanism of Piezo-CAP treatment HCC cells.
Project description:The expression profile of miRNAs during the response of human colon cancer cells (HT29s) to 5-FU treatment and nutrient starvation was examined using miRNA microarray analysis. Combining with the following real time PCR and bioinformatics analysis, the physiological importance of the miRNA-autophagy interconnection is far more likely to be elucidated.
