Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Komagataella phaffii JC308

ABSTRACT: Komagataella phaffii aka Pichia pastoris mutARS-seq, deep mutational scanning of ARSs

PROVIDER: PRJNA223161 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1014260_1.fastq.gz	Fastqsanger.gz
	SRR1014260_2.fastq.gz	Fastqsanger.gz
	SRR1014620_1.fastq.gz	Fastqsanger.gz
	SRR1014620_2.fastq.gz	Fastqsanger.gz
	SRR1014630_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Komagataella phaffii JC308

Project description:Komagataella phaffii aka Pichia pastoris nucleosome mapping

| PRJNA223163 | ENA

Komagataella phaffii JC308

Project description:Komagataella phaffii aka Pichia pastoris ARS-seq, sequencing of ARS plasmids

| PRJNA223160 | ENA

Komagataella phaffii JC308

Project description:Komagataella phaffii Pichia pastoris replication timing profile

| PRJNA223162 | ENA

Komagataella phaffii JC308

Project description:Komagataella phaffii miniARS-seq, sequencing of ARS plasmids

| PRJNA223159 | ENA

A single Gal4-like transcription factor activates the Crabtree effect in Komagataella phaffii

Project description:The Crabtree phenotype defines whether a yeast can perform simultaneous respiration and fermentation under aerobic conditions at high growth rates, a phenomenon that resembles the Warburg effect in cancer cells. Whole genome duplication, global promoter rewiring and loss of respiratory complex I are the main molecular events that contributed to the evolution of Crabtree effect. Here we show that overexpression of a single Gal4-like transcription factor is sufficient to convert Crabtree-negative Komagataella phaffii (Pichia pastoris) into a Crabtree positive yeast. We report the transcriptome profile (RNASeq) of the Δgal4-like and Gal4-like overexpression K. phaffii strains. Upregulation of the glycolytic genes and a significant increase in glucose uptake rate due to the overexpression of the Gal4-like transcription factor caused an overflow metabolism, triggering both short-term and long-term Crabtree phenotypes. This indicates that a single mutation leading to overexpression of one gene may have been sufficient as a first molecular event towards respiro-fermentative metabolism in the course of yeast evolution.

2018-10-19 | GSE110624 | GEO

Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii

Project description:Prevention of COVID-19 on a global scale will require the continued development of high-volume, low-cost platforms for the manufacturing of vaccines to supply on-going demand. Vaccine candidates based on recombinant protein subunits remain important because they can be manufactured at low costs in existing large-scale production facilities that use microbial hosts like Komagataella phaffii (Pichia pastoris). Here, we report an improved and scalable manufacturing approach for the SARS-CoV-2 spike protein receptor binding domain (RBD); this protein is a key antigen for several reported vaccine candidates. We genetically engineered a manufacturing strain of K. phaffii to obviate the requirement for methanol-induction of the recombinant gene. Methanol-free production improved the secreted titer of the RBD protein by >5x by alleviating protein folding stress. Removal of methanol from the production process enabled scale up to a 1,200 L pre-existing production facility. This engineered strain is now used to produce an RBD-based vaccine antigen that is currently in clinical trials and could be used to produce other variants of RBD as needed for future vaccines.

2021-12-15 | GSE183408 | GEO

Komagataella phaffii

Project description:Komagataella phaffii SNP analysis

| PRJNA592463 | ENA

Crosslinking Mapping of yeast Komagataella phaffii TFIID complex

Project description:Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) providing regulatory and chromatin binding functions. We used CXMS to study the organization and structure of the purified TFIID complex from Komagataella phaffii. Together with Cryo-EM, We confirm the unique subunit stoichiometry prevailing in TFIID and uncover a hexameric arrangement of Tafs containing a HF domain. Interaction with promoter DNA highlights two non-selective binding sites consistent with a DNA scanning mode.

2018-10-08 | PXD011092 | Pride

Komagataella phaffii

Project description:Komagataella phaffii Raw sequence reads

| PRJNA930494 | ENA

Komagataella phaffii

Project description:Komagataella phaffii Raw sequence reads

| PRJNA1157989 | ENA