Podospora anserina
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA223302 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptomic response of Podospora anserina to bacterial and fungal non self
2017-03-01 | GSE78796 | GEO
Project description:A whole genome microarray approach has been engaged in the heterothallic euascomycete Podospora anserina to identify genes that are differentially expressed beetwen a wild type kinetics of sexual reproduction and two mutant strains ∆RID and ∆SMR1.
2019-09-16 | GSE104632 | GEO
Project description:A whole genome microarray approach has been engaged in the heterothallic euascomycete Podospora anserina to identify genes that regulate the key steps of its sexual development. A time-course transcriptional study of 10 developmental stages from fertilization to ascospore maturation has been performed. More than half of the genome (58% of the 10540 CDS) displayed a significant change of transcript levels between at least two developmental stages of the kinetic (i.e. on all comparisons between the 10 developmental stages).
2025-06-30 | GSE93094 | GEO
Project description:The long-term submerged cultivation of Podospora anserina
| PRJNA473312 | ENA
Project description:Abstract In Podospora anserina, the two zinc cluster proteins RSE2 and RSE3 are essential for the expression of the gene encoding the alternative oxidase (aox) when the mitochondrial electron transport chain is impaired. In parallel they activated the expression of gluconeogenic genes encoding phosphoenolpyruvate carboxykinase (pck) and fructose-1,6-biphosphatase (fbp). Orthologues of these transcription factors are present in a wide range of filamentous fungi and no other role than the regulation of these three genes has been evidenced so far. In order to better understand the function and the organisation of RSE2 and RSE3, we conducted a saturated genetic screen based on the constitutive expression of the aox gene. We identified 10 independent mutations in 9 positions in rse2 and 11 in 5 positions in rse3. Deletions were generated at some of these positions and the effects analyzed. This analysis suggests the presence of central regulatory domains and a C-ter activation domain in both proteins. Microarray analysis revealed 598 genes that were differentially expressed in the strains containing gain- or loss-of-function mutations in rse2 or rse3. It showed that in addition of aox, fbp and pck, RSE2 and RSE3 regulate the expression of genes encoding the alternative NADH dehydrogenase, a Zn2Cys6 transcription factor, a flavohemoglobin and various hydrolases. As a complement to expression data, a metabolome profiling approach revealed that both an rse2 gain-of-function mutation and growth on antimycin result in similar metabolic alterations in amino acids, fatty acids and α-ketoglutarate pools.
2013-10-22 | GSE51360 | GEO
Project description:Podospora anserina mat transcriptome - Podo3
| PRJNA442528 | ENA
Project description:Abstract In Podospora anserina, the two zinc cluster proteins RSE2 and RSE3 are essential for the expression of the gene encoding the alternative oxidase (aox) when the mitochondrial electron transport chain is impaired. In parallel they activated the expression of gluconeogenic genes encoding phosphoenolpyruvate carboxykinase (pck) and fructose-1,6-biphosphatase (fbp). Orthologues of these transcription factors are present in a wide range of filamentous fungi and no other role than the regulation of these three genes has been evidenced so far. In order to better understand the function and the organisation of RSE2 and RSE3, we conducted a saturated genetic screen based on the constitutive expression of the aox gene. We identified 10 independent mutations in 9 positions in rse2 and 11 in 5 positions in rse3. Deletions were generated at some of these positions and the effects analyzed. This analysis suggests the presence of central regulatory domains and a C-ter activation domain in both proteins. Microarray analysis revealed 598 genes that were differentially expressed in the strains containing gain- or loss-of-function mutations in rse2 or rse3. It showed that in addition of aox, fbp and pck, RSE2 and RSE3 regulate the expression of genes encoding the alternative NADH dehydrogenase, a Zn2Cys6 transcription factor, a flavohemoglobin and various hydrolases. As a complement to expression data, a metabolome profiling approach revealed that both an rse2 gain-of-function mutation and growth on antimycin result in similar metabolic alterations in amino acids, fatty acids and M-NM-1-ketoglutarate pools. With GPL10116: 7 conditions each with four biological replicates: wt, rse2Y326D, rse3G642V, rse2Y326D-rse3G642V, M-NM-^Trse2, M-NM-^Trse3 and M-NM-^Trse2-M-NM-^Trse3; common reference is a pool of four conditions M48h, M96h, C48h and C96h ; Conditions are labelled in Cy3 and the common reference in Cy5
2013-10-22 | E-GEOD-51360 | biostudies-arrayexpress
Project description:Podospora anserina species complex in France
| PRJNA1195547 | ENA
Project description:Sexual reproduction is controlled by successive transcriptomic waves in Podospora anserina
| PRJNA359987 | ENA
Project description:Transcriptomic response of Podospora anserina to bacterial and fungal non self
| PRJNA313943 | ENA