ABSTRACT: Global gene expression profiling of guinea pig whole-lung and hindlimb muscles following long-term exposure to cigarette smoke and/or hypoxia
Project description:The guinea pig (cavia porcellus) is an excellent experimental model for translation to many aspects of human physiology and disease yet there is limited experimental information regarding its proteome. In an effort to overcome this gap in our knowledge, we generated a comprehensive spectral library of the guinea pig proteome. Homogenates and tryptic peptide digests were prepared from 16 tissues (brain, colon, duodenum, adipose, kidney, large intestine, liver, lung, ovaries, pancreas, placenta, skeletal muscle, small intestine, stomach, heart, uterus) and subjected to >200 DDA runs. Analysis of >250,000 peptide-spectrum matches resulted in the construction of a library of 73594 peptides corresponding to 7667 proteins.
Project description:Despite intense efforts the molecular understanding of this clinically important extra pulmonary manifestation is still limited. Cigarette smoking is the main risk factor for developing COPD and therefore animal chronic smoking models have been proposed for mechanistic studies and biomarker discovery. The overarching aim of our study was to assess whether GP hindlimb muscles (both oxidative and glycolytic) show a transcriptional response to these exposures, and whether such gene signatures can be correlated to the expression of lung secreted proteins. A cross-sectional microarray study was designed. Hartley guinea pigs were divided into four groups: one group was exposed to cigarette smoking for 3 months (n=3-4); a second group was kept in normoxia for 10 weeks and subsequently placed in an hypoxic environment (12% O2) for two weeks (n=3-4); a third group (n=4) was smoke-exposed for 3 months and to chronic hypoxia the last two smoking weeks; finally a forth group (n=3-4) served as controls remaining in normoxia for the whole study period.
