Project description:Seed developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying seed developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the seeds of seedless Ponkan ‘Huagan No.4’ (Citrus reticulata) (HG) and seeded Ponkan ‘Egan No.1’ (Citrus reticulata) (EG) into nucellus and integument/seed coat tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of EG, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of seed abortion.

2023-05-08 | GSE197716 | GEO

Citrus reticulata strain:Egan No.1 | cultivar:Ponkan mandarin

Project description:Citrus reticulata strain:Egan No.1 | cultivar:Ponkan mandarin Transcriptome or Gene expression

| PRJNA261374 | ENA

Transcriptome analysis of Citrus reticulata ovule early development based on laser capture microdissection

Project description:Ovule developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying ovule developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the ovules of seedless Ponkan ‘Huagan NO.4’ (Citrus reticulata) (MT) and seeded Ponkan ‘Egan NO.1’ (Citrus reticulata) (WT) into nucellus and integument tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of WT, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of ovule abortion.

2024-09-01 | GSE183639 | GEO

Citrus reticulata

Project description:Citrus reticulata Genome sequencing

| PRJNA320985 | ENA

Citrus reticulata

Project description:Citrus reticulata Genome sequencing

| PRJNA573624 | ENA

Citrus reticulata

Project description:Citrus reticulata Genome sequencing

| PRJNA997759 | ENA