Project description:Sfp1p is known to form the [ISP+] prion in Saccharomyces cerevisiae. Interestingly enough, the consequences and phenotypes of Sfp1p prionization and its absence are quite different. In order to understand the origin of this difference, we compared transcription profiles of an [ISP+], sfp1delta strains against the control strain (SFP1 [isp-]).
Project description:We report change in the nucleosome occupancy and accessibility upon deletion of ATP-dependent chromatin remodellers (ISW1, ISW2 & CHD1) in Saccharomyces cerevisiae.
Project description:Sfp1p is known to form the [ISP+] prion in Saccharomyces cerevisiae. Interestingly enough, the consequences and phenotypes of Sfp1p prionization and its absence are quite different. In order to understand the origin of this difference, we compared transcription profiles of an [ISP+], sfp1delta strains against the control strain (SFP1 [isp-]). 3 strains (an [ISP+], [isp-] and sfp1delta derivative of 25-25-2V-P3982, Peterhoff genetic collection), each in quadruplicate (4 biological replicates).
Project description:In this study, we used Saccharomyces cerevisiae to investigate the effects of GRX deletion on yeast chronological life span (CLS). Deletion of Grx1 or Grx2 shortened yeast CLS. Quantitative proteomics revealed that GRX deletion increased cellular ROS levels to activate Ras/PKA signal pathway. Our results provided new insights into mechanisms underlying aging process.
Project description:We report change in the chromatin contacts upon deletion of ATP-dependent chromatin remodellers (ISW1, ISW2 and CHD1) in Saccharomyces cerevisiae.
Project description:We report change in the chromatin contacts at nucleosomal resolution upon deletion of ATP-dependent chromatin remodellers(Isw1,Isw2 and Chd1) in Saccharomyces cerevisiae.
Project description:Saccharomyces cerevisiae is an excellent microorganism for industrial succinic acid production, but high succinic acid concentration will inhibit the growth of Saccharomyces cerevisiae then reduce the production of succinic acid. Through analysis the transcriptomic data of Saccharomyces cerevisiae with different genetic backgrounds under different succinic acid stress, we hope to find the response mechanism of Saccharomyces cerevisiae to succinic acid.
Project description:To understand the gene expression in Saccharomyces cerevisiae under fermentative and respiraotry conditions, we perfomred the genome-wide gene expression profiling for the log-phase cells of S. cerevisiae wild type, sef1 deletion, and hyperactive SEF1-VP16 mutants under the YPD and YPGly conditions.
Project description:The goal of these experiments was to define the targets of Ty3 transposition in Saccharomyces cerevisiae. Ty3 is a retroviruslike element that is found at the transcription initiation site of chromosomal tRNA genes.
