Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA227160 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
2011-02-19 | GSE20463 | GEO
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression. Two-condition experiment, Normoxic MSCs vs. Hypoxic MSCs.
2011-02-19 | E-GEOD-20463 | biostudies-arrayexpress
Project description:High altitude environments are characterized by the unique and unavoidable stress of chronic hypoxia. While much is known about gene expression responses to acute or in vitro hypoxia, less is known about the gene expression profiles of animals exposed to systemic chronic hypoxia, such as that experienced at high elevations. Here we simulated the hypoxic environment of two high altitude elevations,and a third chamber recieved ambient Reno air. Mice were housed in the hypoxic chambers for 32 days. We used microarrays to characterize the differential gene expression in the livers of mice housed in hypoxic environment of 4500 m versus 3000 and 1400 m. We used this data to draw hypotheses related to novel physiological responses to chronic systemic hypoxia
2009-04-30 | GSE15891 | GEO
Project description:High altitude environments are characterized by the unique and unavoidable stress of chronic hypoxia. While much is known about gene expression responses to acute or in vitro hypoxia, less is known about the gene expression profiles of animals exposed to systemic chronic hypoxia, such as that experienced at high elevations. Here we simulated the hypoxic environment of two high altitude elevations,and a third chamber recieved ambient Reno air. Mice were housed in the hypoxic chambers for 32 days. We used microarrays to characterize the differential gene expression in the livers of mice housed in hypoxic environment of 4500 m versus 3000 and 1400 m. We used this data to draw hypotheses related to novel physiological responses to chronic systemic hypoxia Experiment Overall Design: Mice were housed one of three chambers; the first received ambient Reno air (1400 m) and the other two received air mixed with nitrogen such that one chamber simulated the hypoxic environment of 3000 m and the third chamber simulated hypoxic environment of 4500 m. Twelve mice were housed in each chamber for 32 days. Liver were extracted, and RNA from livers of 4 mice were pooled such that each treatment was represented by 3 pooled samples. Eight of the nine arrays were used for data analysis; one array was excluded as several Affymetrix quality control metrics indicated data quality that was not reliable.
2009-05-11 | E-GEOD-15891 | biostudies-arrayexpress
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
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Project description:In this study, we employed an integrated proteomic and metabolomic approach to systematically analyze the proteome and metabolome profiles of SHRs following prolonged exposure to a high-altitude hypoxic environment. Bioinformatics and enrichment analysis revealed that proteins with significant differential expression were predominantly involved in pathways such as oxidative phosphorylation, thermogenesis, TCA cycle, and carbon metabolism. Further analysis indicated that alterations in key metabolites, including thiamine, S-adenosylhomocysteine, pantothenic acid, fumaric acid, and homoserine, interact with differentially expressed proteins, collectively contributing to the modulation of blood pressure regulation in SHRs under hypoxic conditions. This research provides new molecular insights into understanding the impact of high-altitude hypoxia on the blood pressure regulatory mechanisms in spontaneously hypertensive rats.
2024-08-27 | PXD055443 |
Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs.
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Project description:Subjects were exposed to three hypoxic environments, a hypobaric altitude chamber (AC), a restricted oxygen breathing device (ROBD), and a restricted oxygen breathing environment (ROBE), and blood was collected during each exposure for total RNA analysis. Microarrays were used to examine the transcriptional response from subjects for each of the three conditions and results were compared. Few differences were noted in gene expresssion when comparing AC, ROBD, and ROBE.
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