Mus musculus
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ABSTRACT:
PROVIDER: PRJNA227739 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:To investigate whether Rbfox3 could alter the expression level of miRNAs during neuronal differentiation of P19 cells, we performed miRNA microarray analysis using the RNAs extracted from untreated (undifferentiated) P19-GFP, RA-treated (neuronally differentiated) P19-GFP, or RA-treated P19-T2 cells. Total 9 samples were analyzed. We compared expression levels of P19-GFP (-) vs P19-GFP (+) vs P19-T2 (+) to identify miRNAs which had changes in expression levels with p < 0.01. From this miRNA list, we compared among P19-GFP (-) vs P19-GFP (+) vs P19-T2 (+) to identify the miRNAs which appeared to correlate with Rbfox3 expression.
2014-09-21 | E-GEOD-52377 | biostudies-arrayexpress
Project description:To investigate whether Rbfox3 could alter the expression level of miRNAs during neuronal differentiation of P19 cells, we performed miRNA microarray analysis using the RNAs extracted from untreated (undifferentiated) P19-GFP, RA-treated (neuronally differentiated) P19-GFP, or RA-treated P19-T2 cells.
2014-09-21 | GSE52377 | GEO
Project description:The direct conversion of human skin fibroblasts to neurons has a low efficiency and unclear mechanism. Here, we show that the knockdown of PTBP2 (nPTB) significantly enhanced the transdifferentiation induced by ASCL1, MiR124-9/9* and p53 shRNA to generate mostly GABAergic neurons. Longitudinal RNAseq analyses identified the continuous induction of many RNA Splicing Regulators (RSRs). Among these, the knockdown of RBFOX3, which encodes the mature neuronal marker NeuN, significantly abrogated the transdifferentiation. Overexpression of RBFOX3 significantly enhanced the conversion induced by AMp; the enhancement was occluded by PTBP2 knockdown. We found that PTBP2 attenuation significantly favored neuron-specific alternative splicing (AS) of many genes involved in synaptic transmission, signal transduction, and axon formation. RBFOX3 knockdown significantly reversed the effect, while RBFOX3 overexpression enhanced it. The study reveals the critical role of neuron-specific AS in the direct conversion of human skin fibroblasts to neurons by showing that PTBP2 attenuation enhances this mechanism in concert with RBFOX3.
2023-10-12 | GSE210131 | GEO
Project description:Analysis of senataxin effect on neuronal differentiation and neurite growth in RA-treated P19 cells by modulating senataxin expression levels. Senataxin silencing does not grossly affect the gene expression profiles of P19 cells At day one total RNA from Setx shRNA P19 cells, wild type human Setx P19 transfected cells with and without retionoic acid were used to compare gene expression profiling versus P19 control cells
2010-07-10 | E-GEOD-21724 | biostudies-arrayexpress
Project description:Analysis of senataxin effect on neuronal differentiation and neurite growth in RA-treated P19 cells by modulating senataxin expression levels. Senataxin silencing does not grossly affect the gene expression profiles of P19 cells
2010-07-10 | GSE21724 | GEO
Project description:LIN28 is an RNA-binding protein expressed in many developing tissues. It can block let-7 microRNA processing and help promote pluripotency. We observe LIN28 expression in the developing neural tube, colocalizing with SOX2, suggesting a role in neural development. To better understand its normal developmental function, we investigated LIN28 activity during neurogliogenesis in vitro where the succession of neuronal to glial cell fates occurs as it does in vivo. LIN28 expression was high in undifferentiated cells, and was down-regulated rapidly upon differentiation. Constitutive LIN28 expression caused a complete block of gliogenesis and an increase in neurogenesis. LIN28 expression was compatible with neuronal differentiation and did not increase proliferation. LIN28 caused significant changes in gene expression prior to any effect on let-7, notably on Igf2. Furthermore, a mutant LIN28 that permitted let-7 accumulation was still able to completely block gliogenesis. Thus, at least two biological activities of LIN28 are genetically separable and may involve distinct mechanisms. LIN28 can differentially promote and inhibit specific fates and does not function exclusively by blocking let-7 family miRNAs. Importantly, LIN28âs role in cell fate succession in vertebrate cells is analogous to its activity as a developmental timing regulator in C. elegans. Two repeats of two cell lines induced to differentiate with retinoic acid/aggregation for four days. Control cells: unmodified mouse P19 embryonal carcinoma cells. Experimental cells: P19 cells constitutively expressing human LIN28A.
2010-03-03 | E-GEOD-19705 | biostudies-arrayexpress
Project description:Transcriptomic, epigenetic and chromatin changes during neuronal differentiation
| PRJDB8286 | ENA
Project description:Effect of depletion of Chd5 on gene expression during neuronal differentiation.
| PRJNA858654 | ENA
Project description:P19 cells, a pluripotent embryonal cell line derived from mouse teratocarcinoma, can be stimulated with retinoic acid to induce neural differentiation forming P19 neurons (McBurney et al., 1982). P19 cells (undifferentiated cells abbreviated as UD-P19 cells) and P19 neurons were cultured in a defined medium lacking fetal bovine serum and conditioned medium was collected. Exosomes were purified from conditioned medium by ultracentrifugation method. Exosomes were characterized using a number of techniques according to the guidelines of ISEV. RNA was purified from exosomes using miRCURY™ RNA isolation kit (Exiqon, Inc.; now Qiagen) according to the instructions of the Supplier. Exosomal RNA concentration was measured using NanoDrop™ 8000 spectrophotometer. The approximate size of exosomal RNAs was analyzed on BioAnalyzer and by 5' end labeling and their separation on denaturing gel. Differential expression of RNAs in exosomes from P19 cells and P19 neurons was determined by RNA-Seq method. Double stranded barcoded cDNA libraries were generated and sequenced on Illumina NextSeq 500 platform. Three biological replicates were used from P19 cells. P19 neurons exosomes were from P19 neurons at 8, 10, 12 days of differentiation. RNA-Seq data was analyzed using bioinformatics pipeline.
2023-02-27 | GSE152655 | GEO