Project description:We report the transcriptomic comparisions between key processes required for various stages of fungal carnivory in nematode-trapping fungus Arthrobotrys oligospora when induced with nematodes. The reference assembly used for remapping is A. oligospora TWF154 (GenBank assembly accession: GCA_004768765.1)
Project description:We report the transcriptomic comparisions between ku70 control and ste12 mutant strains in nematode-trapping fungus Arthrobotrys oligospora when induced with nematodes. Fungal Ste12 transcription factor and the upstream MAPK cascade are highly conserved and plays a role in host sensing and pathogenesis in various fungal pathogens. Identification of Ste12-dependent in A. oligospora may provide further insights into the molecular mechanisms of nematode-sensing and trap morphogenesis. The reference assemly used for remapping is A. oligospora TWF154 (GenBank assembly accession: GCA_004768765.1)
2020-10-24 | GSE159974 | GEO
Project description:Meloidogyne hapla and Medicago truncatula Transcriptome or Gene expression
| PRJNA229407 | ENA
Project description:Arthrobotrys oligospora infecting Caenorhabditis briggsae Transcriptome or Gene expression
Project description:NHR-66, a nuclear hormone receptor in C. elegans, regulates cuticle collagen genes important for fungal adhesion. RNA-seq of wild-type and nhr-66 STOP-IN mutants exposed to Arthrobotrys oligospora revealed >1200 genes downregulated in mutants, including over 40% of the collagen gene family. GO analysis highlighted enrichment in cuticle structure terms.
Project description:We have infected the model legume Medicago truncatula with Meloidogyne hapla and harvested tissue over a time course. Transcriptome sequencing was performed on each sample using the Illumina RNA-Seq method. [Longitudinal Experiment] RNA was isolated from M. hapla eggs and pre-penetration juveniles (J2) and also from a time course of M. truncatula infected with M. hapla J2 at five time points: 1, 2, 4, 5, and 7 days after inoculation (DAI). Roots (local) and shoots (global) tissues from infected and uninfected plants were sampled. Each sample was loaded on five lanes for sequencing. Collectively, 22 samples were generated in total from - M. hapla egg and J2 (two samples), - infected M. truncatula root 1-2-4-5-7 DAI (five samples), - infected M. truncatula shoot 1-2-4-5-7 DAI (five samples), - uninfected M. truncatula root 1-2-4-5-7 DAI (five samples), - and uninfected M. truncatula shoot 1-2-4-5-7 DAI (five samples). Thus, 110 fastq files (= 22 samples x 5 lanes). [Diunrnal Experiment] M. truncatula roots inoculated with M. hapla was sampled at 6 time-points: 22:30, 2:00, 5:00, 6:30, 14:00, and 21:00. Lighting was turned off at 22:00 and turned on at 06:00. Four biological replicates were taken for each time point, providing 24 samples in total. Thus, 24 fastq files (= 6 time points x 4 replicates).
Project description:Infection of the plant parasitic nematode Meloidogyne hapla by the nematode-trapping fungus Arthrobotrys dactyloides. Transcriptome or Gene expression
| PRJNA230433 | ENA
Project description:Transcriptome of AOL_s00215g414 gene mutant strain in Arthrobotrys oligospora