Project description:Genome-wide transcriptome analysis of genetic pertubations induced in Drosophila notum by the over-expression of the transcription factors Achaete, Asense, E(spl)m3-HLH and Senseless.
Project description:Genome-wide transcriptome analysis of genetic pertubations induced in Drosophila notum by the over-expression of the transcription factors Achaete, Asense, E(spl)m3-HLH and Senseless. Transient over-expression of GFP (control), Achaete (Ac), Senseless (Sens), Asense (Ase) and E(spl)m3-HLH transcription factors in Drosophila notum using sca-Gal4, tub-Gal80ts. Transcription factorshave been over-expressed alone or in combinations. Three biological replicates were performed for each conditions.
Project description:Expression profiling of Drosophila mir-8 homozygous mutant pupae at a single developmental stage (72 hours APF). Homozygous mutant and wild type pupae were collected at 72 hours APF (After Puparium Formation). Three independent collections were performed. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together to custom cDNA arrays (DGC1 and 2 EST collections).
