ABSTRACT: Global transcriptome analysis reveals major roles for protein quality control, autophagy, and reactive oxygen species scavenging during the acquisition of desiccation tolerance in the resurrection plant Boea hygrometrica
Project description:Transcriptional profiling of resurrection plant Boea hygrometrica comparing control untreated plants with plants treated with dehydration. Goal was to determine the different effects of desiccation with different rates, and drought acclimation on global gene expression in Boea hygrometrica .
Project description:Transcriptional profiling of resurrection plant Boea hygrometrica comparing control untreated plants with plants treated with dehydration. Goal was to determine the different effects of desiccation with different rates, and drought acclimation on global gene expression in Boea hygrometrica . 8 conditions comparison: untreated plants vs. plants treated with rapid dehydration for 2h (RWC~80%) and 48h (RWC<10%), untreated plants vs.plants treated with slow dehydration for 5d (RWC~80%) and 14d (RWC<10%), untreated plants vs. drought acclimated plants treated with rapid dehydration for 2h (RWC~80%) and 48h (RWC<10%). Biological replicates: 3 control replicates, 3 treated replicates.
Project description:Gene transcript abundances were analyzed with samples taken from hydrated, moderate dehydration (70% RWC) and desiccated (10% RWC) leaf tissues of resurrection plant species B. hygrometrica by using RNA-Seq. Totally, 9888 genes were identified as differentially expressed genes.The results provided insight for exploring the mechanisms of desiccation tolerance.
Project description:Gene transcript abundances were analyzed with samples taken from desiccated (10% RWC), rehydrated to 50% RWC (RE50% RWC) and rehydrated to 100% RWC (RE100% RWC) leaf tissues of resurrection plant species B. hygrometrica by using RNA-Seq. Totally, 10207 genes were identified as differentially expressed genes.The results provided insight for exploring the mechanisms of desiccation tolerance.
Project description:Gene transcript abundances were analyzed with samples taken from hydrated, moderate dehydration (70% RWC) and desiccated (10% RWC) leaf tissues of resurrection plant species B. hygrometrica by using RNA-Seq. Totally, 9888 genes were identified as differentially expressed genes.The results provided insight for exploring the mechanisms of desiccation tolerance. Examination of mRNA transcript abundances in hydrated, dehydrating to 70% RWC and dehydrating to 10% RWC leaf tissues, for each treatment, three biological replicates were included.
