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Arabidopsis thaliana

ABSTRACT: Root transcriptomes for the 19 parents of the Arabidopsis thaliana MAGIC lines

PROVIDER: PRJNA231089 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1046910.fastq.gz	Fastqsanger.gz
	SRR1046920.fastq.gz	Fastqsanger.gz
	SRR1046930.fastq.gz	Fastqsanger.gz
	SRR1046940.fastq.gz	Fastqsanger.gz
	SRR1046911.fastq.gz	Fastqsanger.gz

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Root transcriptomes for the 19 parents of the Arabidopsis thaliana MAGIC lines

Project description:We produced RNA-Seq reads from messenger RNA isolated from root tissue for the 19 MAGIC founder accessions (inbred strains) of Arabidopsis thaliana (see Gan et al. 2011. Nature 477:419-23 for a description of the MAGIC genetic mapping resource). The read data was generated with biological replication (two replicates). The resulting RNA-Seq data provide a resource to assess root gene expression across different accessions of A. thaliana. Comparable RNA-Seq read data for the MAGIC founder accessions for aerial seedling tissue has previously been released under GEO series GSE30720 (Gan et al. 2011. Nature, 477:419-23). Examination of RNA expression in root for the 19 Arabidopsis thaliana accessions used as parents for the MAGIC lines (parental accessions are Bur-0, Can-0, Col-0, Ct-1, Edi-0, Hi-0, Kn-0, Ler-0, Mt-0, No-0, Oy-0, Po-0, Rsch-4, Sf-2, Tsu-0, Wil-2, Ws-0, Wu-0, Zu-0).

2013-12-11 | E-GEOD-53198 | biostudies-arrayexpress

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Project description:We have sequenced messenger RNA isolated from seedling tissue for 19 accessions of Arabidopsis thaliana (with biological replication). The 19 accessions for which RNA-Seq reads were collected have served as the founders for the MAGIC lines, a high-resolution recombinant inbred line mapping resource. RNA sequencing data was used to examine differential gene expression among the accessions.

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Project description:The 19 parent lines of the Arabidopsis thaliana MAGIC collection show significant differences in their root exudate profiles. Changes in metabolism were analyzed by ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time–of–flight mass spectrometry (UPLC-ESI-QTOF-MS) untargeted in positive and negative mode, and targeted for a set of known metabolites.

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