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Mus musculus

ABSTRACT: Expression data from miR-92 over-expressing R26MER/MER mouse embryonic fibroblast (MEFs)

OTHER RELATED OMICS DATASETS IN: E-GEOD-53225

PROVIDER: PRJNA231168 | ENA |

REPOSITORIES: ENA

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E-GEOD-53225

Project description:Expression data from miR-92 over-expressing R26MER/MER mouse embryonic fibroblast (MEFs)

2015-07-31 | E-GEOD-53225 | ExpressionAtlas

Expression data from miR-92 over-expressing R26MER/MER mouse embryonic fibroblast (MEFs)

Project description:miR-92 enhances c-Myc induced apoptosis. In the R26MER/MER mouse embryonic fibroblasts (MEFs), a switchable variant of Myc, MycERT2, was knocked into the genomic region downstream of the constitutive Rosa26 promoter, allowing acute activation of c-Myc by 4-OHT-induced nuclear translocation. This in vitro system nicely recapitulates c-Myc-induced apoptosis, as activated MycERT2 induces strong p53-dependent apoptosis in response to serum starvation. Enforced miR-92 expression in three independent R26MER/MER MEF lines significantly enhanced Myc-induced apoptosis. We used microarrays to investigate the molecular mechanism underlying miR-92 functions.

2013-12-11 | GSE53225 | GEO

Expression data from miR-92 over-expressing R26MER/MER mouse embryonic fibroblast (MEFs)

2013-12-11 | E-GEOD-53225 | biostudies-arrayexpress

miR-17-92/106b regulate beta cell mitotic checkpoint and insulin secretion

Project description:Adult beta cells in the pancreas are the sole source of insulin in our body. Beta cell loss or increased demand for insulin, impose metabolic challenges because adult beta cells are generally quiescent and infrequently re-enter the cell division cycle. miR-17-92/106b is a family of proto-oncogene microRNAs, that regulate proliferation in normal tissues and in cancer. Here, we employ mouse genetics to demonstrate a critical role for miR-17-92/106b in glucose homeostasis and in controlling insulin secretion. Mass spectrometry analysis was performed on miR-17-92LoxP/LoxP;106-25-/- MEF lysate, without or with CRE-Adenovirus. miR-17-92LoxP/LoxP;106-25+/+ MEFs with GFP-Adenovirus served as controls. We demonstrate that miR-17-92/106b regulate the adult beta cell mitotic checkpoint and that miR-17-92/106b deficiency results in reduction in beta cell mass in-vivo. Furthermore, protein kinase A (PKA) is a new relevant molecular pathway downstream of miR-17-92/106b in control of adult beta cell division and glucose homeostasis. Therefore, contributes to the understanding of proto-oncogene miRNAs in the normal, untransformed endocrine pancreas, and illustrates new genetic means for regulation of beta cell mitosis and function by non-coding RNAs.

2019-06-17 | PXD012610 | Pride

Transient DNMT3L expression in mouse embryonic fibroblast (MEFs)

Project description:Transient DNMT3L expression in mouse embryonic fibroblast (MEFs)

| PRJNA558078 | ENA

Gene expression profiling of dsDNA90 or ssDNA90 stimulated Mouse Embryonic Fibroblasts

Project description:Gene expression analysis of wild type, STING knock-out and STAT1 knock-out Mouse Embryonic Fibroblasts (MEFs) stimulated with 90-mer dsDNA or 90-mer ssDNA. Genes whose expression that are affected by cytosolic DNA in a STING dependent manner will be identified and signaling pathways regulated by STING will be elucidated.

2013-01-15 | GSE43481 | GEO

Mus musculus

Project description:Expression data from mouse embryo fibroblast (MEFS)

| PRJNA143247 | ENA

The role of microRNA miR-17-92 in MYC oncogene addiction

Project description:miR-17-92 mediates the MYC oncogene addiction in a conditional mouse lymphoma model. To identify targets of miR-17-92 in this model, miR-17-92 was expressed in the conditional lymphoma cell lines using MSCV-puro. Both control and miR-17-92-expressing conditional lymphoma cell lines were treated with doxycycline (DOX) (20ng/ml) for 48 hours to shut off MYC expression.

2014-05-29 | E-GEOD-57507 | biostudies-arrayexpress

Site-specific cytosine-5 methylation by the mouse RNA methyltransferases Dnmt2 and NSun2 promotes tRNA stability and protein synthesis

Project description:Mouse embryonic fibroblast (MEFs) cell lines and liver samples from Dnmt2 and Nsun2 single mutant, double mutant and wildtype mice were used to identify potential mRNA substrates of both proteins. MEFs were derived from day 13.5 embryos and immortalized by transfection with a plasmid expressing the SV40 large-T antigen. Total RNA of MEFs cell line at passage 9 and total RNA extracted from 2 months old mouse liver tissues of Dnmt2, Nsun2 single mutant, double mutant and wildtype mice were arrayed on Illumina MouseRef8 v2 chips.

2012-07-03 | E-GEOD-36918 | biostudies-arrayexpress

49a825c3-18bb-44ea-a4bc-f1afcc86c4ad - samples

Project description:Organoid cultures derived from colorectal adenomas were transduced with a miR-17-92 expressing vector. RNA from miR17-92-overexpressing organoids and respective non-transduced organoids (controls) was isolated for expression analysis.

| EGAD00001008480 | EGA

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