Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA231307 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:4 mutants-Transcriptome sup62 and cpsf30 mpk4
| PRJNA278715 | ENA
Project description:au12-06_cpsf30 - cpsf30 mips1 transcriptome - How a mutation in the CPSF30 is able to suppress the cell death phenotype observed in the mips1-1 Arabidopsis mutant? - All the plants were cultivated in the same hanging conditions 10days in "short Days" then in long Days. The takings were later made 4days in "long Days" what corresponds to the appearance of the symptoms of cellular death at the mutant mips1-1. On the other hand, the gene CPSF30 codes for two transcribed. We have a double mutant mips1 cpsf30 complemented with the transcribed young.
2014-02-01 | GSE53247 | GEO
Project description:au12-06_cpsf30 - cpsf30 mips1 transcriptome - How a mutation in the CPSF30 is able to suppress the cell death phenotype observed in the mips1-1 Arabidopsis mutant? - All the plants were cultivated in the same hanging conditions 10days in "short Days" then in long Days. The takings were later made 4days in "long Days" what corresponds to the appearance of the symptoms of cellular death at the mutant mips1-1. On the other hand, the gene CPSF30 codes for two transcribed. We have a double mutant mips1 cpsf30 complemented with the transcribed young. 6 dye-swap - genotype comparaison
2014-02-01 | E-GEOD-53247 | biostudies-arrayexpress
Project description:Specific control of Arabidopsis BAK1- and SERK4-regulated cell death by protein glycosylation
| PRJNA294262 | ENA
Project description:Identification of Core Genes Regulating Plant Programmed Cell Death (PCD)
| PRJNA97043 | ENA
Project description:Cell cycle and cell death interaction in plants: PCNA, AtXRs and AtMIPS pathway.
| PRJNA114879 | ENA
Project description:au13-05_sup62 - 4 mutants - Transcriptome analysis of the mutant sup62, cpsf30, two suppressors of programmed cell death in A. thaliana - Plants Col-0, mips1, sup62 and sup62 mips1 were grown for 3 weeks in Short-Day condition and subsequently transfered in Long-Day condition. Rosette leaves were sampled after 4 days in Long-Day condition. 8 dye-swap - gene knock out,genotype comparaison
2015-04-01 | E-GEOD-67010 | biostudies-arrayexpress
Project description:AU13_15_sup62 - 4 mutants - Transcriptome analysis of the mutant sup62, cpsf30, two suppressors of programmed cell death in A. thaliana - Plants Col-0, mips1, sup62 and sup62 mips1 were grown for 3 weeks in Short-Day condition and subsequently transfered in Long-Day condition. Rosette leaves were sampled after 4 days in Long-Day condition.
2015-04-01 | GSE67010 | GEO
Project description:Activation of auxin signalling counteracts photorespiratory hydrogen peroxide dependent cell death
| PRJNA226106 | ENA
Project description:As the most ubiquitous internal modification of eukaryotic mRNA, m6A (N6-methyladenosine) modification plays a vital role in almost every aspect of mRNA metabolism. However, the evidence of m6A in regulating the alternative polyadenylation (APA) is limited. APA is controlled by a large protein-RNA complex with many components including CPSF30. Arabidopsis CPSF30 has two isoforms, the longer one named CPSF30-L containing an additional YTH (YT512-B Homology)-domain in the C terminus, which is unique in plant. In this study, we revealed that m6A modification directly regulated APA, by proving the capability of Arabidopsis CPSF30-L YTH domain binding to m6A substrate by using in vitro assay and structural studies. We observed that extensive genes shifted their poly(A) sites in cpsf30-2 and found that numerous genes altered polyadenylation site (PAS) that correlated well with previously identified m6A peaks, indicating that genes carry m6A modification are prone to be regulated by APA. Moreover, we found that several important genes involved in nitrate metabolism are amongst those genes with APA site alteration in cpsf30-2.And we could rescue these APA and nitrate metabolism defects by introducing the wild-type CPSF30-L, but not by m6A-binding defective mutants (W259A, W310A or Y319A), which explained the nitrogen signaling defects of cpsf30-2 discovered previously. Taken together, our results demonstrated that m6A modification could directly regulate APA in Arabidopsis and revealed the function of m6A reader CPSF30-L in nitrate signaling by controlling APA regulation. This study will shed new lights on the roles of m6A modification during RNA 3’-end processing in plant development.
2021-03-16 | GSE145109 | GEO