Project description:The purpose of this study was to determine the miRNA expression profile of in vitro differentiation of human skeletal muscle cells and to couple changes in individual miRNA expression to effects on target genes by transcriptome profiling of mRNA expression. mRNA expression profiling at three different time points during the in vitro differentiation process of human skeletal muscle cells from three subjects. RNA was harvested from myoblasts before and 4 and 10 days after induction of differentiation. Temporal, time-course design with paired analysis (3 subjects). Biological replicates: 3 at day 0, 3 at day 4, and 3 at day 10. One replicate per array.
Project description:The purpose of this study was to determine the miRNA expression profile of in vitro differentiation of human skeletal muscle cells and to couple changes in individual miRNA expression to effects on target genes by transcriptome profiling of mRNA expression. mRNA expression profiling at three different time points during the in vitro differentiation process of human skeletal muscle cells from three subjects. RNA was harvested from myoblasts before and 4 and 10 days after induction of differentiation.
Project description:The purpose of this study was to determine the miRNA expression profile of in vitro differentiation of human skeletal muscle cells and to couple changes in individual miRNA expression to transcriptional output of target genes. miRNA expression profiling at six different time points during the in vitro differentiation process of human skeletal muscle cells from six subjects. RNA was harvested from myoblasts before induction of differentiation and at every other day for 10 following days. Temporal, time-course design with paired analysis (6 subjects). Biological replicates: 6 at day 0, 6 at day 2, 6 at day 4, 6 at day 6, 6 at day 8, and 5 at day 10. One replicate vs common reference RNA pool per array.
Project description:Skeletal muscle has been identified as a secretory organ. We hypothesized that IL-6, a cytokine secreted from skeletal muscle during exercise, could induce production of other secreted factors in skeletal muscle. Keywords: Human skeletal muscle time course response to IL-6 infusion
Project description:Skeletal muscle has been identified as a secretory organ. We hypothesized that IL-6, a cytokine secreted from skeletal muscle during exercise, could induce production of other secreted factors in skeletal muscle. Keywords: Human skeletal muscle time course response to IL-6 infusion IL-6 was infused for 3 h into healthy young males (n=7) and muscle biopsies obtained at time points 0, 3 and 6 h in these individuals. Affymetrix microarray analysis of gene expression profiling in muscle were carried out on 3 randomly chosen subjects.