Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Characterization of the Merkel cell carcinoma miRNome

PROVIDER: PRJNA232473 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1057530.fastq.gz	Fastqsanger.gz
	SRR1057523.fastq.gz	Fastqsanger.gz
	SRR1057524.fastq.gz	Fastqsanger.gz
	SRR1057525.fastq.gz	Fastqsanger.gz
	SRR1057526.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Homo sapiens

Project description:Merkel Cell Carcinoma Cell Lines - RNA-seq

| PRJNA503609 | ENA

Homo sapiens

Project description:Epigenome signature of merkel cell carcinoma

| PRJNA671514 | ENA

Homo sapiens

Project description:Comparative Transcriptional Profiling of Human Merkel cells and Merkel Cell Carcinoma

| PRJNA217485 | ENA

Homo sapiens

Project description:Gene expression analysis of Merkel Cell Carcinoma

| PRJNA128667 | ENA

Homo sapiens

Project description:microRNA expression patterns related to merkel cell polyomavirus infection in human merkel cell carcinoma

| PRJNA187073 | ENA

Homo sapiens

Project description:Array Comparative Genomic Hybridization of Merkel Cell Carcinoma Tumors

| PRJNA109513 | ENA

Crystal structure of Homo sapiens kynureninase.

Project description:Kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5'-phosphate (PLP) dependent enzymes known as the aspartate aminotransferase superfamily or alpha-family. The Homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. The human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacterial expression system using Ni metal ion affinity chromatography. Kinetic characterization of the recombinant enzyme reveals classic Michaelis-Menten behavior, with a Km of 28.3 +/- 1.9 microM and a specific activity of 1.75 micromol min-1 mg-1 for 3-hydroxy-dl-kynurenine. Crystals of recombinant kynureninase that diffracted to 2.0 A were obtained, and the atomic structure of the PLP-bound holoenzyme was determined by molecular replacement using the Pseudomonas fluorescens kynureninase structure (PDB entry 1qz9) as the phasing model. A structural superposition with the P. fluorescens kynureninase revealed that these two structures resemble the "open" and "closed" conformations of aspartate aminotransferase. The comparison illustrates the dynamic nature of these proteins' small domains and reveals a role for Arg-434 similar to its role in other AAT alpha-family members. Docking of 3-hydroxy-l-kynurenine into the human kynureninase active site suggests that Asn-333 and His-102 are involved in substrate binding and molecular discrimination between inducible and constitutive kynureninase substrates.

| S-EPMC2531291 | biostudies-literature

Comparative Transcriptional Profiling of human Merkel cells and Merkel Cell Carcinoma

Project description:Merkel cell carcinoma is supposed to be derived from Merkel cells after infection by Merkel cell polyomavirus (MCV) and other poorly known events. A transcriptional profiling with cDNA microarrays was performed on cells from MCV+ Merkel cell carcinomas and isolated normal Merkel cells. This microarray revealed numerous significantly upregulated genes and down-regulated genes. The extensive list of genes identified in these experiments provides a large body of potentially valuable information of Merkel cell carcinoma carcinogenesis and could represent a source of potential targets for cancer therapy. Two-conditions experiment, MCV vs Normal Merkel Cell. Biological replicates : 4 MCV (Cy5), 1 control = pool of Normal Merkel cells from 3 liftings

2014-01-01 | E-GEOD-50434 | biostudies-arrayexpress

Array Comparative Genomic Hybridization of Merkel Cell Carcinoma Tumors

Project description:Array-CGH profiles of Merkel cell carcinoma tumors Keywords: fresh frozen and formalin fixed paraffin embedded primary tumor, nodal and metastasis merkel cell carcinoma tumor samples

2009-01-01 | GSE13239 | GEO

Homo sapiens

Project description:Gene expression changes associated with prognosis of Merkel cell carcinoma

| PRJNA152891 | ENA