Project description:Recent developments in high-throughput RNA sequencing (RNA-seq) technology have had a dramatic impact on our comprehension of the structure and expression profiles of the mammalian transcriptome. This study performed transcriptome analysis of Rongchang pig brains and livers using deep RNA-seq technology to offer insights into potential swine genetic improvements, in addition to examining the use of the Rongchang pig as a biomedical model.By sequencing the mRNA of two extreme tissues, brains and livers, a total of 8.6 Gb of sequencing was obtained, with 91% resulting in clean reads, with approximately 43 million reads from the brain and 44 million reads from the liver samples. This analysis revealed tissue specificity through the identification of 5,575 and 4,600 differentially expressed genes (DEGs) in brains and livers respectively. Functional analysis of the differentially expressed genes showed that the top ten functional pathways with highly enriched gene expression in brains were associated with nerves, while in livers the pathways were predominantly related to metabolism. Furthermore, 83 neuropeptide gene transcripts, 69 neuropeptide receptor gene transcripts, 10 pro-neuropeptide convertase gene transcripts, 12 insulin-like growth factors binding protein gene transcripts, and other neuropeptide related protein gene transcripts were identified, to reveal different expression profiles in brain and liver samples. In this study, major characteristics of the transcriptional profiles of Rongchang pig brains and livers were present. Overall, our data provides useful information for future research pertaining to swine livestock and biomedical research.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples
Project description:To obtain an overview of the transcriptome landscape in developing pig skeletal muscle, 81 high-quality transcriptome libraries that covered 27 developmental stages (3 biological replicates per stage) in pig skeletal muscle were produced by strand-specific rRNA-depleted total RNA sequencing (RNA-seq). We generated 8.59 billion paired-end reads (150 bp × 2) covering 1.24 Tb of sequence for RNA-seq.
Project description:We have employed aCGH analysis of tumor tissue compared to non-tumourous tissue in pigs treated with diethylnitrosamine (DENA). Beside other liver tumors angiosarcoma-like tumors were observed in the DENA-treated livers.