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Populus trichocarpa

ABSTRACT: Ptr-miR397a is a negative regulator of laccase genes affecting lignin content in Populus trichocarpa

OTHER RELATED OMICS DATASETS IN: E-GEOD-54153

PROVIDER: PRJNA235309 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1121300.fastq.gz	Fastqsanger.gz
	SRR1121301.fastq.gz	Fastqsanger.gz
	SRR1121292.fastq.gz	Fastqsanger.gz
	SRR1121302.fastq.gz	Fastqsanger.gz
	SRR1121293.fastq.gz	Fastqsanger.gz

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Similar Datasets

Ptr-miR397a is a negative regulator of laccase genes affecting lignin content in Populus trichocarpa

Project description:Laccases were proposed to catalyze the oxidative polymerization of monolignols. We identified 49 laccase gene models in Populus trichocarpa, of which 29 were predicted to be targets of ptr-miR397a. We overexpressed Ptr-MIR397a in transgenic P. trichocarpa. In each of all 9 transgenic lines tested, 17 PtrLACs were down-regulated as analyzed by RNA-seq. Transgenic lines with severe reduction in the expression of these laccase genes resulted in an approximately 40% decrease in the total laccase activity. Overexpression of Ptr-MIR397a in these transgenic lines also reduced lignin content, whereas levels of all monolignol biosynthetic gene transcripts remained unchanged. A hierarchical genetic regulatory network (GRN) built by a bottom-up Graphic Gaussian Model algorithm provides additional support for a role of ptr-miR397a as a negative regulator of laccases for lignin biosynthesis. Full transcriptome based differential gene expression in the overexpressed transgenics and protein domain analyses implicate previously unidentified transcription factors and their targets in an extended hierarchical GRN including ptr-miR397a and laccases that coregulate lignin biosynthesis in wood formation. Ptr-miR397a, laccases and other regulatory components of this network may provide additional strategies for genetic manipulation of lignin content. Total twelve trees were used. Those include nine individual transgenic trees for overexpressing Ptr-miR397a, as nine biological replicates, and three wild-type trees.

2014-01-17 | E-GEOD-54153 | biostudies-arrayexpress

Ptr-miR397a is a negative regulator of laccase genes affecting lignin content in Populus trichocarpa

2014-01-17 | GSE54153 | GEO

Transcriptome analyses of the Laccaria bicolor-Populus trichocarpa mycorrhiza

Project description:Illumina HiSeq2000 technology was used to generate mRNA profiles from the ectomycorrhizal fungi Laccaria bicolor colonizing roots of Populus trichocarpa. Samples were taken after 3 months of contact in order to identify mycorrhiza-regulated transcripts. 100bp reads were generated and aligned to the Populus trichocarpa (http://www.phytozome.net/poplar.php) reference genome.

2017-04-03 | GSE75437 | GEO

RNAseq of bending treated Populus trichocarpa

Project description:We treated 6 month Populus trichocarpa by bending for 0, 3, and 7 days to see effects of bending treatment on wood formation at transcript level

2020-07-05 | GSE153793 | GEO

Transcriptome analyses of the Laccaria bicolor-Populus trichocarpa mycorrhiza development

2014-02-08 | GSE54789 | GEO

Ozone exposure of Populus deltoides and Populus trichocarpa

Project description:Populus deltoides and Populus trichocarpa were exposed to either ambient air or an acute ozone exposure of 200 ppb for 9 hrs and ozone response was profiled for each genotype by hybridising control against ozone-exposed samples per genotype. Keywords: stress response, genotype comparrison, ozone exposure

2009-04-01 | GSE10874 | GEO

Gene expression in leaves of a Populus trichocarpa and Populus deltoides pseudo-backcross population

Project description:A microarray analysis of whole-genome gene expression in leaves was carried out in a (Populus trichocarpa X Populus deltoides) X Populus deltoides pseudo-backcross pedigree. Genetic variation in gene expression was quantified for 55,793 predicted gene models based on a single probe per gene. Resultant data contributed to the analysis of the genetic architecture of gene expression in leaves of Populus.

2010-02-02 | GSE20117 | GEO

Gene expression in whole-roots of a Populus trichocarpa and Populus deltoides pseudo-backcross population

Project description:A microarray analysis of whole-genome gene expression in roots was carried out in a (Populus trichocarpa X Populus deltoides) X Populus deltoides pseudo-backcross pedigree. Genetic variation in gene expression was quantified for 55,793 predicted gene models based on a single probe per gene. Resultant data contributed to the analysis of the genetic architecture of gene expression in roots of Populus.

2010-02-02 | GSE20118 | GEO

RNAseq of xylem tissue of transgenic and wildtype Populus trichocarpa (NSF Plant Genome Research Program Project 0922391)

Project description:We transformed Populus trichocarpa and generated transgenics with knockdown or overexpression of monolignol genes and transcription factors

2016-03-07 | GSE78953 | GEO

Transcriptome analyses of the Laccaria bicolor-Populus trichocarpa mycorrhiza development

Project description:Illumina GAIIx technology was used to generate mRNA profiles from the ectomycorrhizal fungi Laccaria bicolor colonizing roots of Populus trichocarpa. Samples were taken after two, four and 12 weeks of contact in order to identify mycorrhiza-regulated transcripts. 37bp reads were generated and aligned to the Populus trichocarpa (http://www.phytozome.net/poplar.php) and the Laccaria bicolor (http://genome.jgi-psf.org/Lacbi2/Lacbi2.home.html) reference genomes using CLC Genomics Workbench 6. mRNA profiles from Populus trichocarpa roots colonized by Laccaria bicolor for two, four and 12 weeks as well as from control roots and free-living mycelium were generated by using one lane of 37 bp Illumina GAIIx sequencing per sample.

2014-02-08 | E-GEOD-54789 | biostudies-arrayexpress

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