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Francisella tularensis subsp. novicida U112

ABSTRACT: Francisella tularensis subsp. novicida U112 Genome sequencing

PROVIDER: PRJNA236529 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
		Other
	SRR2891721_1.fastq.gz	Fastqsanger.gz
	SRR2891721_2.fastq.gz	Fastqsanger.gz
	SRR2891722.fastq.gz	Fastqsanger.gz
	SRR2891723_subreads.fastq.gz	Fastqsanger.gz

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In vivo screen of Francisella tularensis subsp. novicida mutants in sequence defined transposon library

Project description:We used an inhalation mouse model of infection to query a collection of 2149 mutants in a Francisella tularensis subsp. novicida background for genes required for growth, survival and systemic dissemination. A microarray-based genome-wide negative selection screen (Microarray tracking of transposon mutants = MATT) allowed us to monitor the behavior of transposon insertions in 1371 unique genes. Interestingly most of these genes persisted in lung and colonized liver and spleen. We found 44 (35%) genes negatively selected in lung and 81 (65%) genes negatively selected in liver and/or spleen. If negative selection in lung occurred, the attenuated mutants in general persisted at 24h after infection, disseminated to liver and/or spleen and appeared to be lost in lung after 48 to 72h of infection. These genes with a strong phenotype in lung but also potential for dissemination might be attractive vaccine or drug candidates. Keywords: Genome-Wide Negative Selection Screen

2008-12-31 | GSE11297 | GEO

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