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Arabis alpina

ABSTRACT: Genome-wide profiling of H3K4me3, H3K27me3 H3K27me1 and 5mC enrichment and mRNA levels in young leaves of Arabis alpina

PROVIDER: PRJNA237036 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1151360_1.fastq.gz	Fastqsanger.gz
	SRR1151360_2.fastq.gz	Fastqsanger.gz
	SRR1151351.fastq.gz	Fastqsanger.gz
	SRR1151361_1.fastq.gz	Fastqsanger.gz
	SRR1151361_2.fastq.gz	Fastqsanger.gz

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Genome-wide profiling of H3K4me3, H3K27me3 H3K27me1 and 5mC enrichment and mRNA levels in young leaves of Arabis alpina

Project description:Chromatin, DNA and RNA were extracted from young A. alpina Pajares primary stem leaves. Chromatin and DNA methylation immunoprecipitation experiments were performed using commercially available antibodies and analyzed by Illumina sequencing (ChIP-seq and MeDIP-seq). Transcriptome data were generated by RNA-seq. ChIP-seq analysis of H3K4me3, H3K27me3 and H3K27me1 enrichment profiles and MeDIP-seq analysis of 5mC enrichment profiles in 2 biological replicates. RNA-seq analysis of mRNA levels in 1 biological replicate.

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Project description:Transcriptomic profile of Arabis alpina apex enriched material. We compared apices from plants grown for 2 weeks in long days (20M-BM-0C) (juvenile) with apices from plants grown for 8 weeks in long days (20M-BM-0C) (adult) and apices from plants grown for 2 weeks and then shifted in vernalization for 4 weeks with apices from plants grown for 8 weeks and than shifted to vernalization for 4 weeks. 2x2 factorial design. 2 starting ages (2 weeks and 8 weeks) and 2 developmental conditions (before and after vernalization). 4 biological replicates for each condition (16 arrays). Reference: pool of the 16 arrays.

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