Project description:The leaf transcriptome of the nickel hyperaccumulator specie Noccaea caerulescens subsp. fimiensis living on serpentine soils were compared to the closely related non-nickel-accumulators Noccaea caerulescens "Viviez" growing on a zinc mining site and Noccaea montana living on serpentine soil, to identity differentially expressed genes potentially involved in Ni hyperaccumulation.
Project description:In this study, we assess the DNA damage occurring in response to cadmium (Cd) in the Cd hyperaccumulator Noccaea caerulescens Ganges (GA) vs the non-accumulator and close-relative species Arabidopsis thaliana. At this purpose, the alkaline comet assay was utilized to evaluate the Cd-induced variations in nucleoids and the methy-sens comet assay, and semiquantitative real-time (qRT)-PCR were also performed to associate nucleus variations to possible DNA modifications. Cadmium induced high DNA damages in nuclei of A. thaliana while only a small increase in DNA migration was observed in N. caerulescens GA. In addition, in N. caerulescens GA, CpG DNA methylation increase upon Cd when compared to control condition, along with an increase in the expression of MET1 gene, coding for the DNA-methyltransferase. N. caerulescens GA does not show any oxidative stress under Cd treatment, while A. thaliana Cd-treated plants showed an upregulation of transcripts of the respiratory burst oxidase, accumulation of reactive oxygen species, and enhanced superoxide dismutase activity. These data suggest that epigenetic modifications occur in the N. caerulescens GA exposed to Cd to preserve genome integrity, contributing to Cd tolerance.
Project description:Root transcriptomes were sequenced using SOLiD 4 chemistry. The three accessions LC, GA and MP differed significantly in the expression of genes that contribute to metal ion transport and detoxification, stress tolerance and secondary metabolite biosynthesis. These data provide comprehensive genome-wide view to the root processes in several N. caerulescens accessions and highlight differences that potentially facilitate adaptation to various metalliferous soils. Comparison of three N. caerulescens accessions with contrasting metal accumulation and tolerance traits
Project description:Root transcriptomes were sequenced using SOLiD 4 chemistry. The three accessions LC, GA and MP differed significantly in the expression of genes that contribute to metal ion transport and detoxification, stress tolerance and secondary metabolite biosynthesis. These data provide comprehensive genome-wide view to the root processes in several N. caerulescens accessions and highlight differences that potentially facilitate adaptation to various metalliferous soils.
Project description:Difference in gene expression profiles between the two metal hyperaccumulators Noccaea japonica and Noccaea caerulescens ecotype Ganges under excess nickel condition
Project description:Here we compare mineral accumulation and global gene expression patterns between two metal hyperaccumulator plants - Noccaea japonica, originating from Ni-rich serpentine soils, and Noccaea caerulescens (ecotype Ganges), originating from Zn/Pb-mine soils - under excess Ni conditions. Significant differences in the accumulation of K, P, Mg, B, and Mo were explained by the expression levels of specific transporters for each mineral. We previously showed that total Ni accumulation in the whole plant is higher in N. caerulescens than in N. japonica. Here we found a similar tendency for Fe under excess Ni; however, the expression of iron-regulated transporter 1 (IRT1), which encodes the primary Fe uptake transporter and causes excess Ni uptake in Arabidopsis thaliana, was higher in N. japonica. NjIRT1 has a point mutation at Asp100, which is essential for Fe transport, and so might lack its Fe and possibly Ni transport function. Noccaea japonica might have lost its IRT1 function, which would prevent excess Ni uptake via IRT1 in Ni-rich soils, and come to rely on other transporters.