Project description:To investigate and compare the influence of root exudates of tomato and maize on Pseudomonas donghuensis P482, we have grown the strain up to a stationary phase in M9 0.4% glucose medium supplemented with maize exudates (Maize), tomato exudates (Tomato) or without supplementation (Control). We then performed differential gene expression analysis, identifying changes in transcriptome profiles between each treatment (Tomato, Maize) and the Control as reference conditions, and between the two treatments.
Project description:We screened a number of virulence genes in Pseudomonas donghuensis HYS, and genes in cbr/crc regulatory system were included. To investigate the relationship between this regulatory system and other virulence genes screened in the study, we performed transcriptome sequencing of strains related to cbr/crc system. Through analyzing the expression quantity, we found that the screened virulence genes were under the regulation of the cbr/crc system.
