ABSTRACT: 16S rRNA Gene Pyrosequencing of groundwater and surrogate sediment samples during HRC injection for chromium reduction at the 100-H site, Hanford, Washington, USA
Project description:A marine bacterial isolate, Brevibacterium casei #NIOSBA88 was grown separately in Luria Bertini broth supplemented with 750 mg L-1 Arsenic (III) and 100 mg L-1 Chromium respectively. Similarly, the strain was grown in absence of Arsenic and Chromium, which served as an experimental control. The cells were harvested at different time intervals and proteins were extracted from the cell pellets using in-house protocol (Shah and Damare 2018). The extracted proteins were digested using trypsin following standard protocol. Tryptic digests were injected in LCMS QToF for obtaining spectral library which was used to identify the proteins against a species specific database in NCBI.
Project description:Columns containing Hanford 100H aquifer sediment continuously infused with 5 mM lactate, 5 uM Cr(VI), and either 7.5 mM sulfate or 12 mM nitrate as an electron acceptor.
Project description:Peripheral blood NK cells were cultured into either control or cytokine-induced memory-like NK cells as described in PMID: 32614951. After differentiation, cells were labeled with hashtag antibodies and cite-seq antibodies and control and memory-like NK cells were combined and loaded onto the 10x Genomics Chromium Instrument a the McDonnell Genome Institute at Washington University School of Medicine. The following samples were analyzed to develop a classification tool for unbiased identification of memory-like NK cells in a bulk single-cell RNA-seq population.
Project description:This dataset contains single nucleus Assay for Transposase-Accessible Chromatin (ATAC) sequencing results from rat nucleus accumbens tissue. Rats received repeated cocaine injections (20mg/kg, intraperitoneal injection), or saline injections as a control. Single-nucleus ATAC-seq was carried out with FACS-sorted nuclei using the 10X Genomics Chromium single cell sequencing platform using the Chromium Next GEM Single Cell ATAC Kit v2.
Project description:We investigate changes in the single-cell transcriptomic profile of mouse (C57BL/6) splenocytes at late stage of Trypanosoma evansi Merzouga 93 (ITMAS 150399C) infection. Infection was initiated by Intraperitoneal injection with parasites diluted in PBS. On day 42 post-injection, mice spleens were disassociated, RBC lysed to obtain the cells for 10x Chromium 3' Gene Expression Profiling
Project description:We investigate changes in the single-cell transcriptomic profile of mouse (C57BL/6) splenocytes during Trypanosoma Evansi Merzoua-93 (ITMAS 150399C) infection. Infection was initiated by Intraperitoneal injection with parasites diluted in PBS. On day 0/14/42 post-injection, mice spleens were disassociated, RBC lysed to obtain the cells for 10x Chromium 3' Gene Expression Profiling
