Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Plant metagenome

ABSTRACT: Fungal ITS1 amplicon library from surface sterilized plants and lichens

PROVIDER: PRJNA239523 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1183466.fastq.gz	Fastqsanger.gz

Items per page:

1 - 1 of 1

Similar Datasets

plant metagenome

Project description:Willow root fungal endophytic metagenome, ITS1 amplicons

| PRJNA716888 | ENA

uncultured fungus

Project description:Fungal ITS1 amplicon library from environmental samples Targeted Locus (Loci)

| PRJNA168235 | ENA

Project description:Fungal ITS1 amplicon from environmental samples

| PRJNA464273 | ENA

Diverse plant RNAs coat Arabidopsis leaves and are distinct from apoplastic RNAs

Project description:Transgenic expression of a double-stranded RNA in plants can induce silencing of homologous mRNAs in fungal pathogens. Although such host-induced gene silencing is well documented, the molecular mechanisms by which RNAs can move from the cytoplasm of plant cells across the plasma membrane of both the host cell and fungal cell are poorly understood. Indirect evidence suggests that this RNA transfer may occur at a very early stage of the infection process, prior to breach of the host cell wall, suggesting that silencing RNAs might be secreted onto leaf surfaces. To assess whether Arabidopsis plants possess a mechanism for secreting RNA onto leaf surfaces, we developed a protocol for isolating leaf surface RNA separately from intercellular (apoplastic) RNA. This protocol yielded abundant leaf surface RNA that displayed an RNA banding pattern distinct from apoplastic RNA, suggesting that it may be secreted directly onto the leaf surface rather than exuded through stomata or hydathodes. Notably, this RNA was not associated with either extracellular vesicles or protein complexes; however, RNA species longer than 100 nucleotides could be pelleted by ultracentrifugation. Furthermore, pelleting was inhibited by the divalent cation chelator EGTA, suggesting that these RNAs may form condensates on the leaf surface. These leaf surface RNAs are derived almost exclusively from Arabidopsis, but come from diverse genomic sources, including rRNA, tRNA, mRNA, intergenic RNA, microRNAs, and small interfering RNAs, with tRNAs especially enriched. We speculate that endogenous leaf surface RNA plays an important role in the assembly of distinct microbial communities on leaf surfaces.

2024-12-14 | GSE283977 | GEO

PLANT METAGENOME

Project description:Diversity of Weed Endophytic Fungi: ITS1 and ITS2 survey

| PRJNA622886 | ENA

Indoor plant microbiome

Project description:Microbial communities of indoor plants - Amplicon Sequencing

| PRJEB38711 | ENA

plant metagenome

Project description:Metagenomic analysis of endophytic fungal diversity associated with leaf samples of medicinal plants Memecylon umbellatum

| PRJNA646829 | ENA

Project description:fungal ITS1 genes

| PRJNA606021 | ENA