Project description:This experiment aims to study transcriptional alterations induced by reconstitution of wild type E-cadherin expression in human lobular breast cancer cells harbouring deleterious, somatic CDH-1 mutations Two cell lines (IPH-926 and MDA-MB-134) expressing synthetic Ecad (EcadEGFP) or not (EGFP) were compared against each other. Analysis were conducted in triplicates (IPH-926) and quadruplicates (MDA-MB-134), respectively.
Project description:We report the genome-wide chromatin binding profiles of ERα and phosphorylated CREB1 (pCREB1) upon stimulation of human MDA-MB-134 breast cancer cells with estradiol or cAMP. We highlight the unique and the shared cistromes for each transcription factor.
