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Bovine gut metagenome

ABSTRACT: Super shedders of Escherichia coli O157:H7 have a unique fecal bacterial community structure

PROVIDER: PRJNA241439 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1201650.fastq.gz	Fastqsanger.gz
	SRR1201660.fastq.gz	Fastqsanger.gz
	SRR1201651.fastq.gz	Fastqsanger.gz
	SRR1201642.fastq.gz	Fastqsanger.gz
	SRR1201652.fastq.gz	Fastqsanger.gz

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bovine gut metagenome

Project description:Changes in bacterial community composition of Escherichia coli O157:H7 super-shedding cattle occur in the lower intestine

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Project description:Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a notorious foodborne pathogen capable of causing severe gastrointestinal infections in humans. The bovine rectoanal junction (RAJ) has been identified as a primary reservoir of STEC O157:H7, playing a critical role in its transmission to humans through contaminated food sources. Despite the relevance of this host-pathogen interaction, the molecular mechanisms behind the adaptation of STEC O157:H7 in the bovine RAJ and its subsequent infection of human colonic epithelial cells remain largely unexplored. This study aimed to unravel the intricate dynamics of STEC O157:H7 in two distinct host environments: bovine RAJ squamous epithelial (RSE) cells and human colonic epithelial cells. Comparative transcriptomics analysis was employed to investigate the differential gene expression profiles of STEC O157:H7 during its interaction with these cell types. The bacterial cells were cultured under controlled conditions to simulate the microenvironments of both bovine RAJ and human colonic epithelial cells. Using high-throughput RNA sequencing, we identified key bacterial genes and regulatory pathways that are significantly modulated in response to each specific host environment. Our findings reveal distinct expression patterns of virulence factors, adhesion proteins, and stress response genes in STEC O157:H7 grown in bovine RAJ cells as opposed to human colonic epithelial cells. Additionally, the comparative analysis highlights the potential role of certain genes in host adaptation and tissue-specific pathogenicity. Furthermore, this study sheds light on the potential factors contributing to the survival and persistence of STEC O157:H7 in the bovine reservoir and its ability to colonize and cause disease in humans.

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