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Diplodus puntazzo

ABSTRACT: Diplodus puntazzo Transcriptome or Gene expression

PROVIDER: PRJNA241484 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1197942_1.fastq.gz	Fastqsanger.gz
	SRR1197942_2.fastq.gz	Fastqsanger.gz
	SRR1197966_1.fastq.gz	Fastqsanger.gz
	SRR1197966_2.fastq.gz	Fastqsanger.gz
	SRR1202032_1.fastq.gz	Fastqsanger.gz

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Diplodus puntazzo

Project description:Diplodus puntazzo overview

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Diplodus puntazzo

Project description:Diplodus puntazzo Raw sequence reads

| PRJNA611935 | ENA

Pathogens of Diplodus puntazzo larvae

Project description:Investigation of epitheliocystis pathogens of Diplodus puntazzo larvae directly from infected tissue samples

| PRJEB7440 | ENA

Caretta caretta / Diplodus puntazzo

Project description:2bRAD sequences of Caretta caretta and Diplodus puntazo.

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The sex-specific transcriptome of the hermaphrodite sparid sharpsnout seabream (Diplodus puntazzo).

Project description:BackgroundTeleosts are characterized by a remarkable breadth of sexual mechanisms including various forms of hermaphroditism. Sparidae is a fish family exhibiting gonochorism or hermaphroditism even in closely related species. The sparid Diplodus puntazzo (sharpsnout seabream), exhibits rudimentary hermaphroditism characterized by intersexual immature gonads but single-sex mature ones. Apart from the intriguing reproductive biology, it is economically important with a continuously growing aquaculture in the Mediterranean Sea, but limited available genetic resources. Our aim was to characterize the expressed transcriptome of gonads and brains through RNA-Sequencing and explore the properties of genes that exhibit sex-biased expression profiles.ResultsThrough RNA-Sequencing we obtained an assembled transcriptome of 82,331 loci. The expression analysis uncovered remarkable differences between male and female gonads, while male and female brains were almost identical. Focused search for known targets of sex determination and differentiation in vertebrates built the sex-specific expression profile of sharpsnout seabream. Finally, a thorough genetic marker discovery pipeline led to the retrieval of 85,189 SNPs and 29,076 microsatellites enriching the available genetic markers for this species.ConclusionsWe obtained a nearly complete source of transcriptomic sequence as well as marker information for sharpsnout seabream, laying the ground for understanding the complex process of sex differentiation of this economically valuable species. The genes involved include known candidates from other vertebrate species, suggesting a conservation of the toolkit between gonochorists and hermaphrodites.

| S-EPMC4133083 | biostudies-literature

The complete mitochondrial genome of the sharpsnout seabream <i>Diplodus puntazzo</i> (Perciformes: Sparidae).

Project description:The sharpsnout seabream Diplodus puntazzo Walbaum, 1792 is a target species of small-scale fishery activities and is cage-cultured for human consumption. Nonetheless, genetic information on this species is limited. We here first sequence its complete mitochondrial genome. The sequence is composed of 16,638 base pairs, accounting for 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 2 non-coding regions (D-loop and L-origin). The overall nucleotide composition is: 27.4% A, 28.9% C, 26.9% T, and 16.8% G. Maximum likelihood analyses placed D. puntazzo close to Acanthopagrus and some Pagellus species.

| S-EPMC7782139 | biostudies-literature

First report of Edwardsiellosis in cage-cultured sharpsnout sea bream, Diplodus puntazzo from the Mediterranean.

Project description:Edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. The bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic Edwardsiella from those isolated from humans by placing them in a new species, E. piscicida. Here we report the first case of Edwardsiellosis in cultured sharpsnout sea breams, Diplodus puntazzo in Greece.The disease has affected cultured sharpsnout sea breams of a commercial fish farm in a single location in East Greece. Two populations of sharpsnout sea breams stocked in two consecutive years in floating cages presented signs of disease which included nodules and abscesses in spleen and kidney, morbidity and cumulative mortality reaching 5.3 %. Using microbiological, biochemical and molecular tools we have identified Edwardsiella sp. as the main aetiological factor of the disease. Following phylogenetic analysis the bacterial isolates are grouped with the newly described Edwardsiella piscicida species.This is the first report of Edwardsiellosis in this species but most importantly in sea cage-cultured fish in the Mediterranean which may pose a serious threat for aquaculture fish species in this region.

| S-EPMC4508803 | biostudies-literature

fDipPun

Project description:Diplodus puntazzo (Sharpsnout seabream) genomic and transcriptomic data (fDipPun1)

| PRJEB49350 | ENA

Project description:Reservebenefit: Diplodus sargus

| PRJNA716499 | ENA

Biomarks of Resistance to Cetuximab in Colorectal Cancer

Project description:Interventions: A group:Before using cetuximab;B group:After progression of disease:no Primary outcome(s): KRAS gene sequence;NRAS gene sequence;BRAF gene sequence;PIK3CA gene sequence;EGFR gene sequence;EGFR gene copy numbers;EGFR expression;Her2 expression;c-Met expression;PTEN expression Study Design: historical control

| 2671639 | ecrin-mdr-crc

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