Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA244010 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcript Level Expression Profiling: anticancer pathways of Smilax Glabra rhizome extract (SW) [HepG2 cells]
| PRJNA251556 | ENA
Project description:Smilax glabra Roxb, a traditional Chinese herb, has been widely used for folk medicine. Previous studies have found that it has various pharmacological activities, such as cytotoxic, anti-inflammation, anti-oxidant, hepatoprotective, and cardiovascular system protective activities. However, its roles in adipogenesis are poorly understood. We hypothesized that Smilax glabra Roxb and its main component may play important roles in regulating adipocyte diffrentiation and function. To test this hypothesis, we performed RNA-Seq on 3T3-L1 adipocytes treated with and without pure total flavonoids from Smilax glabra Roxb.
2022-01-11 | GSE124950 | GEO
Project description:To find the possible signaling pathways which contribute to the anticancer effect of SW-treated HepG2 cells Global gene expression profiling is an ideal technique to obtain useful clues for exploration of the anticancer mechanism of SW. Through comparing microarray results between solvent- and SW-treated cells, differentially expressed genes were obtained (>1.5 fold). The microarray results were validated using real-time RT-PCR. We used the KEGG database, STRING database and GO database for further ananlysis, and therefore got the possible signaling pathways underlying the anticancer effect of SW.
2014-06-08 | GSE58201 | GEO
Project description:Find the possible signaling pathways which contribute to the cell growth inhibition effect of SW-treated AGS cells Global gene expression profiling is an ideal technique to obtain useful clues for exploration of the anticancer mechanism of SW. Through comparing microarray results between solvent- and SW-treated cells, differentially expressed genes were obtained (>1.5 fold). The microarray results were validated using real-time RT-PCR. We used the KEGG database, STRING database and GO database for further ananlysis, and therefore got the possible signaling pathways underlying the anticancer effect of SW.
2014-04-09 | GSE56593 | GEO
Project description:To find the possible signaling pathways which contribute to the anticancer effect of SW-treated HepG2 cells Global gene expression profiling is an ideal technique to obtain useful clues for exploration of the anticancer mechanism of SW. Through comparing microarray results between solvent- and SW-treated cells, differentially expressed genes were obtained (>1.5 fold). The microarray results were validated using real-time RT-PCR. We used the KEGG database, STRING database and GO database for further ananlysis, and therefore got the possible signaling pathways underlying the anticancer effect of SW. We analyzed total RNA samples of solvent- and SW-treated HepG2 cells using the Affymetrix Human Gene 1.0 ST platform. No techinical replicates were performed.
2014-06-08 | E-GEOD-58201 | biostudies-arrayexpress
Project description:Find the possible signaling pathways which contribute to the cell growth inhibition effect of SW-treated AGS cells Global gene expression profiling is an ideal technique to obtain useful clues for exploration of the anticancer mechanism of SW. Through comparing microarray results between solvent- and SW-treated cells, differentially expressed genes were obtained (>1.5 fold). The microarray results were validated using real-time RT-PCR. We used the KEGG database, STRING database and GO database for further ananlysis, and therefore got the possible signaling pathways underlying the anticancer effect of SW. We analyzed total RNA samples of solvent- and SW-treated AGS cells using the Affymetrix Human Gene 1.0 ST platform. No techinical replicates were performed.
2014-04-09 | E-GEOD-56593 | biostudies-arrayexpress
Project description:Extracts from the rhizome of Cimicifuga racemosa (black cohosh) are increasingly popular as herbal alternative to hormone replacement therapy (HRT) for the alleviation of postmenopausal disorders. However, the molecular mode of action and the active principles are presently not clear. Previously published data have been largely contradictory. We, therefore, investigated the effects of a lipophilic Cimicifuga rhizome extract on the ER+ breast cancer MCF-7 cells at transcriptional level in comparision to 17beta-estradiol and the ER antagonist tamoxifen. With the extract 431 genes were regulated more than 1.5 fold. The overall expression pattern differed from those of 17β-estradiol or the estrogen receptor antagonist tamoxifen. We observed an enrichment of genes in an anti-proliferative and apoptosis-sensitizing manner, together with an increase of mRNAs coding for gene products involved in several stress response pathways. Regulated genes of these functional groups were highly overrepresented among all regulated genes. Various transcripts coding for oxidoreductases were induced, as for example the cytochrome P450 family members 1A1 and 1B1. In addition, some transcripts associated with antitumor but also tumor-promoting activity were regulated. Experiment Overall Design: MCF-7 cells were treated for 24 h with a lipophilic (dichloromethane) Cimicifuga rhizome extract, 17beta-estradiol, tamoxifen and the solvent control (DMSO 0,1%) in duplicate
2008-06-15 | E-GEOD-6800 | biostudies-arrayexpress