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Francisella tularensis subsp. novicida strain:FAI

ABSTRACT: Francisella tularensis subsp. novicida strain: FAI Genome sequencing

PROVIDER: PRJNA244559 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR2891724_1.fastq.gz	Fastqsanger.gz
	SRR2891724_2.fastq.gz	Fastqsanger.gz
	SRR2891725.fastq.gz	Fastqsanger.gz

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Project description:We used an inhalation mouse model of infection to query a collection of 2149 mutants in a Francisella tularensis subsp. novicida background for genes required for growth, survival and systemic dissemination. A microarray-based genome-wide negative selection screen (Microarray tracking of transposon mutants = MATT) allowed us to monitor the behavior of transposon insertions in 1371 unique genes. Interestingly most of these genes persisted in lung and colonized liver and spleen. We found 44 (35%) genes negatively selected in lung and 81 (65%) genes negatively selected in liver and/or spleen. If negative selection in lung occurred, the attenuated mutants in general persisted at 24h after infection, disseminated to liver and/or spleen and appeared to be lost in lung after 48 to 72h of infection. These genes with a strong phenotype in lung but also potential for dissemination might be attractive vaccine or drug candidates. Keywords: Genome-Wide Negative Selection Screen

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