Project description:We report the transcriptomic information of wild type (Lab-WT) A.baumannii 98-37-09 and A1S_3277 transposon mutant during the growth in human serum with 0.15 µg/mL levofloxacin
Project description:Among in silico predicted or experimentally confirmed miR-15a targets are many genes of relevance to AAA pathology and known to be down-regulated in AAA conditions. To better understand which of these targets could be linked to the observed detrimental effects of miR-15a on SMC dynamics in vitro, we performed RNA-sequencing of hAoSMCs transfected with miR-15a-modulators (mimic, inhibitor, and scrambled control). Upon transfection with miR-15a-mimic, 1030 genes were significantly down-regulated and 741 were significantly up-regulated, whereas miR-15a-inhibition led to 23 down-regulated and 82 up-regulated genes.
Project description:We intended to investigate effects of mmu-miR-15a-3p on gene expression in mice We used microarrays to compare gene expression in mouse B/CMBA.Ov cell lines transfected with mmu-miR-15a-3p and negative control mimic
Project description:MicroRNAs are important regulators of gene expression and associated with stress-related psychiatric disorders. We report that exposing mice to chronic stress led to a specific increase in microRNA-15a levels in the amygdala-Ago2 complex, and a concomitant reduction in the levels of its predicted target, FKBP51, which is implicated in stress-related psychiatric disorders. Reciprocally, mice expressing reduced levels of amygdalar microRNA-15a following exposure to chronic stress exhibited increased anxiety-like behaviors. Here, we performed small RNA Sequencing of mouse basolateral amygdala after miR15a knockdown using injection of a miR-15a sponge virus or control sponge virus.
Project description:ChIP-seq was performed in C. elegans to characterize the genomic binding profiles of LIN-35 (DREAM complex), LIN-15B, and LIN-15A, and to assess their interdependencies. Experiments were conducted in wild-type animals and in lin-15A(fp31), lin-15B(n744), and lin-35(n745) null mutants. All three factors showed highly overlapping binding patterns, predominantly at promoter regions, and shared target genes enriched for metabolic and developmental pathways. Comparative analyses revealed reciprocal effects on binding between LIN-35 and LIN-15B, while loss of LIN-15A primarily resulted in increased LIN-35 and LIN-15B occupancy at a subset of loci, suggesting that LIN-15A modulates DREAM complex binding.