Project description:We report the transcriptomic information of wild type (Lab-WT) A.baumannii 98-37-09 and A1S_3277 transposon mutant during the growth in human serum with 0.15 µg/mL levofloxacin
Project description:We intended to investigate effects of mmu-miR-15a-3p on gene expression in mice We used microarrays to compare gene expression in mouse B/CMBA.Ov cell lines transfected with mmu-miR-15a-3p and negative control mimic
Project description:MicroRNAs are important regulators of gene expression and associated with stress-related psychiatric disorders. We report that exposing mice to chronic stress led to a specific increase in microRNA-15a levels in the amygdala-Ago2 complex, and a concomitant reduction in the levels of its predicted target, FKBP51, which is implicated in stress-related psychiatric disorders. Reciprocally, mice expressing reduced levels of amygdalar microRNA-15a following exposure to chronic stress exhibited increased anxiety-like behaviors. Here, we performed small RNA Sequencing of mouse basolateral amygdala after miR15a knockdown using injection of a miR-15a sponge virus or control sponge virus.
Project description:The emerging foodborne pathogen, Aeromonas hydrophila, co-infects humans and animals, especially fish, threatening aquacultural production and public health. Previously we found that Scatophagus argus, a widely cultivated fish species with high economic value, exhibited enhanced growth but increased susceptibility to A. hydrophila infection under freshwater conditions compared to seawater conditions. However, the exact mechanisms involved remain unclear.Our study demonstrated that enhanced virulence of A. hydrophila 201416 isolated from S. argus induced by increasing salinity was associated with altered quorum sensing-related gene expression and regulated behaviors. Results from virulence assays combining phenotypic characterization indicated that increased salinity (from 0 to 35 g/L NaCl) impeded Ah201416 infection of S. argus, a trend aligning with increased biofilm mass and swimming motility, but opposite to bacterial growth. RNA-sequencing and quantitative reverse transcriptional PCR analysis confirmed significant upregulation of genes related to flagellar assembly (flgB, flgH, flgC, flgI, flhA, and fliA), bacterial secretion (HlyD and Ahh1), and quorum sensing (AhyR, LuxO, and LuxE) of Ah201416 in response to elevated salinity. These findings suggested that increased salinity not merely enhanced the virulence of Ah201416 but bolstered the resistance of S. argus, thereby mitigating its susceptibility. This study provides further insights into the microbial risks associated with A. hydrophila in aquacultural production, which is critical to developing appropriate prevention and control strategies and ensuring safe seafood supply.
Project description:While microRNAs (miRs) have been extensively studied in the context of malignancy and tumor progression, their functions in regulating T cell activation are less clear. We found reduced levels of miR-15a/16 at 3-18 h post-T cell receptor (TCR) stimulation, suggesting a role in shaping T cell activation. An inducible miR15a/16 transgenic mouse model was developed to determine how elevating miR-15a/16 levels during early stages of activation would affect T cell proliferation and to identify TCR signaling pathways regulated by this miR pair. Doxycyclin (DOX) induced expression of miR-15a/16 from 0-18 h post-TCR stimulation decreased ex vivo proliferation as well as in vivo antigen-specific proliferation. Bioinformatic and proteomic approaches were combined to identify MEK1 as a target of miR-15a/16. MEK1 targeting by miR-15a/16 was confirmed using miR mimics that decreased MEK1 containing the 3’-UTR target nucleotide sequence (UGCUGCUA) but did not decrease MEK1 containing a mutated control sequence (AAAAAAAA). Phosphorylation of downstream signaling molecules ERK1/2 and Elk1 were decreased with DOX-induced miR-15a/16 expression. In addition to MEK1, ERK1 was subsequently found to be targeted by miR-15a/16, with DOX induced miR-15a/16 reducing total ERK1 levels in T cells. These findings show that TCR stimulation reduces miR-15a/16 levels at early stages of T cell activation to facilitate increased MEK1 and ERK1, and this promotes sustained MEK1-ERK1/2-Elk1 signaling required for optimal proliferation.
Project description:Analysis of mouse chondrocytes lacking the microRNA-140. MicroRNAs are genomically encoded small RNAs to regulate the gene expression. miR-140 shows high expression in cartilage. Results provide insight into the molecular mechanisms underlying miR-140 function in chondrocytes. Keywords: Expression profiling by array