Project description:We tested whether home field advantage at inter- and intra-specific levels alters microbial carbon transformations, using a multi-factorial design with microcosms of freshwater submerged leaf litter from two species (Alder and Hemlock) exposed to 'home' or 'away' communities of microbes isolated from decomposing leaf litter, as well as controls with no microbial community added. For all 'home', 'away' and control conditions for each species we also had high or low oxygen treatments. Samples were taken at day 0, 154, 257 and 354 and extracted with solid phase columns and methanol to provide extracted metabolites.