Schizosaccharomyces pombe
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ABSTRACT:
PROVIDER: PRJNA245908 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Heterochromatin integrity affects chromosome reorganization after centromere dysfunction
| PRJNA108495 | ENA
Project description:Heterochromatin plays a key role in gene repression, maintaining genome integrity and chromosome segregation. Fission yeast, Schizosaccharomyces pombe, utilizes conserved components to direct heterochromatin formation using siRNA generated by RNA interference (RNAi) to guide a histone H3 lysine 9 methyltransferase to cognate chromatin. To identify compounds that inhibit heterochromatin formation, an in vivo phenotypic screen for loss of silencing was performed. A tester strain harbouring a silent dominant selectable kanMX reporter gene within fission yeast centromeric heterochromatin was used to screen a diverse library of chemicals. HMS-I1 and HMS-I2 were identified as compounds that reproducibly increased G418 resistance due to loss of kanMX silencing, and decreased the level of repressive H3K9 methylation on centromeric repeats. The pattern of changes induced by HMS-I1 and HMS-I2 were consistent with inhibition of the histone deacetylases (HDACs) Clr3 and/or Sir2. Chemical-genetic interactions and expression profiling indicated that both HMS-I1 and HMS-I2 affect the activity of the Clr3-containing Snf2/HDAC repressor complex (SHREC). Exposure to HMS-I1 was also found to alleviate silencing of reporter genes in an Arabidopsis transgenic plant line and a mouse cell line. HMS-I2 also disrupted reporter gene silencing in Arabidopsis. In vitro assays indicate that HMS-I1 impairs the activity of human HDAC6 and HDAC10. As HMS-I1 and HMS-I2 bear no resemblance to known inhibitors of chromatin-based activities they represent potentially novel and valuable reagents for experimental and therapeutic purposes. Our findings highlight the use of in vivo chemical screening conducted in fission yeast to identify compounds that disrupt heterochromatin across plant, fungi and animal kingdoms. 16 RNA samples: 2 replicates of WT untreated (vehicle DMSO) (KE1-A, KE2-A), HMS-I1 treated (KE1-B, KE2-B), HMS-I2 treated (KE1-C, KE2-C), and 2 additional replicates of WT (KE05_wt_1, KE10_wt_2), 4 replicates of clr2M-bM-^HM-^F (KE01_2118_1, KE06_2118_2, KE03_0080_1, KE08_0080_2) and mit1M-bM-^HM-^F (KE02_1295_1, KE07_1295_2, KE04_1278_1, KE09_1278_2).
2014-12-17 | E-GEOD-57207 | biostudies-arrayexpress
Project description:Epigenetic adaptation to uncontrolled heterochromatin spreading
| PRJNA258461 | ENA
Project description:Loss of Epe1 enhances ectopic heterochromatin formation
| PRJDB6425 | ENA
Project description:Centromeric H2B monoubiquitination promotes noncoding transcription and chromatin integrity
| PRJNA215328 | ENA
Project description:Heterochromatin plays a key role in gene repression, maintaining genome integrity and chromosome segregation. Fission yeast, Schizosaccharomyces pombe, utilizes conserved components to direct heterochromatin formation using siRNA generated by RNA interference (RNAi) to guide a histone H3 lysine 9 methyltransferase to cognate chromatin. To identify compounds that inhibit heterochromatin formation, an in vivo phenotypic screen for loss of silencing was performed. A tester strain harbouring a silent dominant selectable kanMX reporter gene within fission yeast centromeric heterochromatin was used to screen a diverse library of chemicals. HMS-I1 and HMS-I2 were identified as compounds that reproducibly increased G418 resistance due to loss of kanMX silencing, and decreased the level of repressive H3K9 methylation on centromeric repeats. The pattern of changes induced by HMS-I1 and HMS-I2 were consistent with inhibition of the histone deacetylases (HDACs) Clr3 and/or Sir2. Chemical-genetic interactions and expression profiling indicated that both HMS-I1 and HMS-I2 affect the activity of the Clr3-containing Snf2/HDAC repressor complex (SHREC). Exposure to HMS-I1 was also found to alleviate silencing of reporter genes in an Arabidopsis transgenic plant line and a mouse cell line. HMS-I2 also disrupted reporter gene silencing in Arabidopsis. In vitro assays indicate that HMS-I1 impairs the activity of human HDAC6 and HDAC10. As HMS-I1 and HMS-I2 bear no resemblance to known inhibitors of chromatin-based activities they represent potentially novel and valuable reagents for experimental and therapeutic purposes. Our findings highlight the use of in vivo chemical screening conducted in fission yeast to identify compounds that disrupt heterochromatin across plant, fungi and animal kingdoms.
2014-12-17 | GSE57207 | GEO
Project description:Acetylation and dimethylation of lysine 9 on histone H3 are prominent marks of euchromatin and heterochromatin, respectively. Moreover, histone acetylation has been linked to lipid metabolism. We have previously identified the transcription factor Cbf11 as a regulator of lipid metabolism and genome integrity in the fission yeast. Cut6, the acetyl-CoA carboxylase, is one of direct targets of Cbf11. To link the role of Cbf11 in lipid metabolism and chromatin regulation we have performed ChIP-seq of H3K9ac, H3K9me2 and H3 in WT and cbf11KO strains of Schizosaccharomyces pombe in three biological replicates. Strain genotypes: wild type JB32 (h+s); cbf11 knock-out MP44 (h+ cbf11::natR).
2021-11-03 | E-MTAB-11081 | biostudies-arrayexpress
Project description:S. pombe Sin3 homolog Pst3 is Key in Nucleolar Integrity
| PRJNA98199 | ENA
Project description:Ccp1 homodimer mediates chromatin integrity by antagonizing CENP-A loading
| PRJNA336376 | ENA
Project description:Nuclear aconitase regulates heterochromatin formation by interacting with Chp1
| PRJNA395555 | ENA