Project description:PREX2 truncating mutations occur in melanoma. We used microarray based gene expression profiling to compare expression patterns between cells harboring Suv420h1 knockout and PREX2 mutant expressing Spontaneously immortalized MEFs from either wt or Suv420h1 knockout mice were used. Wt MEFs were transduced with lentivirus to express indicated PREX2 mutants. Suv420h1 ko MEFs were reconstituted either with control GFP or Suv420h1-GFP fusion. Total RNA was isolated and subjected to analysis
Project description:This project have LC-MSMS analysis results of label free quantitation of N-terminome enrichment of mouse proteome. N-terminal modification of mouse embryonic fibroblasts (MEFs) lacking Naa10 show negligible difference between wild-type and Naa10 mutant.
