Project description:We microdissected each compartment from 6-micron paraffin sections using the Leica AS LMD system to identify all genes active in different compartments of a soybean seed containing globular-stage embryos. Experiment Overall Design: Globular-stage seed compartments were isolated using the Leica AS LMD system. Total RNA was amplified and hybridized with Affymetrix Soybean Genome Arrays.
Project description:We performed small-RNA profiling of soybean seed regions and sub-regions, to gain new insights into the regulatory processes required for seed differentiation. Four subregions of the Globular stage of the soybean seeds were analyzed. Two biological replicates were collected for each subregions.
Project description:We report the genome-wide small RNA of soybean early maturation seed coat parenchyma compartment soybean early maturation seeds using Illumina high-throughput sequencing technology. Illumina sequencing of small RNA from early maturation seed coat parenchyma compartment and early-maturation stage whole seeds
Project description:We microdissected each compartment from 6-micron paraffin sections using the Leica AS LMD system to identify all genes active in different compartments of a soybean seed containing globular-stage embryos. Keywords: cell type comparison