Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Caenorhabditis elegans

ABSTRACT: Global characterization of the oocyte-to-embryo transition in C. elegans uncovers a novel mRNA turnover mechanism

PROVIDER: PRJNA251377 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1313060_1.fastq.gz	Fastqsanger.gz
	SRR1313060_2.fastq.gz	Fastqsanger.gz
	SRR1313061_1.fastq.gz	Fastqsanger.gz
	SRR1313061_2.fastq.gz	Fastqsanger.gz
	SRR1313054_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Caenorhabditis elegans

Project description:A novel small molecule that disrupts the oocyte-to-embryo transition in C. elegans

| PRJNA329115 | ENA

Caenorhabditis elegans

Project description:LIN-41 and OMA ribonucleoprotein complexes mediate a translational repression-to-activation switch controlling oocyte meiotic maturation and the oocyte-to-embryo transition in Caenorhabditis elegans

| PRJNA384040 | ENA

The APC/CFZY-1/Cdc20 complex coordinates with OMA-1 to regulate the oocyte-to-embryo transition in C. elegans

Project description:During oocyte maturation and the oocyte-to-embryo transition, key developmental regulators such as RNA-binding proteins coordinate translation of particular mRNAs and related developmental processes by binding to their cognate maternal mRNAs. In the nematode C. elegans, these processes are regulated by a set of CCCH zinc finger proteins. OMA-1 and OMA-2 are two functionally redundant CCCH zinc finger proteins that turnover rapidly during the first embryonic cell division. These turnovers are required for proper transition from oogenesis to embryogenesis. A gain-of-function mutant of OMA-1, oma-1(zu405), stabilizes and delays degradation of OMA-1, resulting in delayed turnover and mis-segregation of other cell fate determinants, which eventually causes embryonic lethality. We performed a large-scale forward genetic screen to identify suppressors of the oma-1(zu405) mutant. We show here that multiple alleles affecting functions of various APC/C subunits, including MAT-1, MAT-2, MAT-3, EMB-30, and FZY-1, suppress the gain-of-function mutant of OMA-1. Mutations in APC/C genes prevent OMA-1 enrichment in P granules and correct delayed degradation of downstream cell fate determinants including PIE-1, POS-1, MEX-3, and MEG-1. Transcriptome analysis also suggested that overall transcription in early embryos occurred after introducing mutations in APC/C genes into the oma-1(zu405) mutant. We demonstrated that only the activator FZY-1, but not FZR-1, is incorporated in the APC/C complex to regulate the oocyte-to-embryo transition. Our findings suggested a genetic relationship linking the APC/C complex and OMA-1, and support a model in which the APC/C complex promotes P granule accumulation and modifies RNA binding of OMA-1 to regulate the oocyte-to-embryo transition process.

2021-10-02 | GSE181115 | GEO

Caenorhabditis elegans

Project description:C elegans N2 Late embryo input control

| PRJNA148537 | ENA

Caenorhabditis elegans

Project description:Global analysis of Caenorhabditis elegans operons

| PRJNA91921 | ENA

Caenorhabditis elegans

Project description:The gastrula transition reorganizes replication origin selection in Caenorhabditis elegans

| PRJNA342483 | ENA

PM2.5 exposure induces lifespan reduction in Caenorhabditis elegans

Project description:To elucidate the molecular mechanism underlying lifespan reduction induced by PM2.5 exposure in Caenorhabditis elegans, we performed global gene expression profiling by RNA-sequencing technology, and compared the gene expression pattern change induced by PM2.5 exposure.

2023-02-22 | GSE214215 | GEO

Caenorhabditis elegans

Project description:Global analysis of dauer gene expression in Caenorhabditis elegans

| PRJNA92707 | ENA

Caenorhabditis elegans

Project description:Global profile of germline gene expression in C. elegans

| PRJNA85207 | ENA

Caenorhabditis elegans

Project description:Global genetic robustness of the alternative splicing machinery in C. elegans

| PRJNA116849 | ENA