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Suppression of progenitor differentiation requires the long noncoding RNA ANCR

ABSTRACT: Suppression of progenitor differentiation requires the long noncoding RNA ANCR

PROVIDER: PRJNA251407 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR403879_1.fastq.gz	Fastqsanger.gz
	SRR403879_2.fastq.gz	Fastqsanger.gz
	SRR403880_1.fastq.gz	Fastqsanger.gz
	SRR403880_2.fastq.gz	Fastqsanger.gz
	SRR403881_1.fastq.gz	Fastqsanger.gz

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Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR

Project description:This SuperSeries is composed of the following subset Series: GSE34528: Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR [HG-U133_Plus_2] GSE34766: Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR [lincRNA 391k Tiling Array V2] Refer to individual Series

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Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR

Project description:Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR

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Suppression of progenitor differentiation requires the long noncoding RNA ANCR.

Project description:Long noncoding RNAs (lncRNAs) regulate diverse processes, yet a potential role for lncRNAs in maintaining the undifferentiated state in somatic tissue progenitor cells remains uncharacterized. We used transcriptome sequencing and tiling arrays to compare lncRNA expression in epidermal progenitor populations versus differentiating cells. We identified ANCR (anti-differentiation ncRNA) as an 855-base-pair lncRNA down-regulated during differentiation. Depleting ANCR in progenitor-containing populations, without any other stimuli, led to rapid differentiation gene induction. In epidermis, ANCR loss abolished the normal exclusion of differentiation from the progenitor-containing compartment. The ANCR lncRNA is thus required to enforce the undifferentiated cell state within epidermis.

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR [HG-U133_Plus_2]

Project description:Long non-coding RNAs (lncRNAs) regulate diverse processes, yet a potential role for lncRNAs in maintaining the undifferentiated state in somatic tissue progenitor cells remains uncharacterized. We used transcriptome sequencing and tiling arrays to compare lncRNA expression in epidermal progenitor populations versus differentiating cells. We identified ANCR (anti differentiation ncRNA) as an 855 bp lncRNA down-regulated during differentiation. Depleting ANCR in progenitor-containing populations, without any other stimuli, led to rapid differentiation gene induction. In epidermis, ANCR loss abolished the normal exclusion of differentiation from the progenitor-containing compartment. The ANCR lncRNA is thus required to enforce the undifferentiated cell state within epidermis. Gene expression analysis: To establish the global gene regulatory effects of ANCR depletion, total RNA was isolated in biologic duplicate from cells in different conditions and hybridized to Affymetrix HG-U133 2.0 Plus arrays.

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Suppression of Progenitor Differentiation Requires the Long Non-Coding RNA ANCR [lincRNA 391k Tiling Array V2]

Project description:Long non-coding RNAs (lncRNAs) regulate diverse processes, yet a potential role for lncRNAs in maintaining the undifferentiated state in somatic tissue progenitor cells remains uncharacterized. We used transcriptome sequencing and tiling arrays to compare lncRNA expression in epidermal progenitor populations versus differentiating cells. We identified ANCR (anti differentiation ncRNA) as an 855 bp lncRNA down-regulated during differentiation. Depleting ANCR in progenitor-containing populations, without any other stimuli, led to rapid differentiation gene induction. In epidermis, ANCR loss abolished the normal exclusion of differentiation from the progenitor-containing compartment. The ANCR lncRNA is thus required to enforce the undifferentiated cell state within epidermis. Custom tiling arrays designed to assay the intergenic space corresponding to regions of H3K4me3-H3K36me3 domains (Khalil et al., 2009) were used to assay changes in RNA expression of putative lncRNAs. RNA from progenitor populations and terminally differentiated populations of human keratinocytes, adipocytes, and osteoblasts were hybridized to these arrays and differential expression was assessed.

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