Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Caenorhabditis elegans

ABSTRACT: LIN-42, the Caenorhabditis elegans PERIOD homolog, negatively regulates microRNA transcription

PROVIDER: PRJNA251425 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1314160.fastq.gz	Fastqsanger.gz
	SRR1314161.fastq.gz	Fastqsanger.gz
	SRR1314162.fastq.gz	Fastqsanger.gz
	SRR1314156.fastq.gz	Fastqsanger.gz
	SRR1314157.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 7

Similar Datasets

Caenorhabditis elegans

Project description:Staufen Negatively Modulates MicroRNA Activity in Caenorhabditis elegans

| PRJNA315193 | ENA

Staufen Negatively Modulates MicroRNA Activity in Caenorhabditis elegans

Project description:The double-stranded RNA-binding protein Staufen has been implicated in various posttranscriptional gene regulatory processes. Here, we demonstrate that the Caenorhabditis elegans homolog of Staufen, STAU-1, functionally interacts with microRNAs. Loss-of-function mutations of stau-1 significantly suppress phenotypes of let-7 family microRNA mutants, a hypomorphic allele of dicer and a lsy-6 microRNA partial loss-of-function mutant. Furthermore, STAU-1 modulates the activity of lin-14, a target of lin-4 and let-7 family microRNAs, and this modulation is abolished when the 3' untranslated region of lin-14 is removed. Deep sequencing of small RNA cDNA libraries reveals no dramatic change in the levels of microRNAs, or other small RNA populations between wild-type and stau-1 mutants, with the exception of certain endogenous siRNAs in the WAGO pathway. The modulation of microRNA activity by STAU-1 does not seem to be associated with the previously reported enhanced exogenous RNAi (Eri) phenotype of stau-1 mutants, since eri-1 exhibits the opposite effect on microRNA activity. Altogether, our results suggest that STAU-1 negatively modulates microRNA activity downstream of biogenesis, possibly by competing with microRNAs for binding on the 3' untranslated region of target mRNAs

2016-03-16 | GSE79217 | GEO

Staufen Negatively Modulates MicroRNA Activity in Caenorhabditis elegans

2016-03-16 | E-GEOD-79217 | biostudies-arrayexpress

Effect of lin-28 and lin-46 on miRNA profile in late L1 stage Caenorhabditis elegans

Project description:Comparison of miRNA profiles of wildtype and lin-28(n719); lin-46(ma164) Caenorhabditis elegans nematodes at the L1 stage

2012-02-09 | GSE35634 | GEO

Caenorhabditis elegans

Project description:MicroRNA-Directed siRNA Biogenesis in Caenorhabditis elegans

| PRJNA124873 | ENA

Caenorhabditis elegans

Project description:Effect of lin-28 and lin-46 on miRNA profile in late L1 stage Caenorhabditis elegans

| PRJNA152485 | ENA

Caenorhabditis elegans

Project description:Caenorhabditis elegans

| PRJNA265871 | ENA

LIN-42, the Caenorhabditis elegans PERIOD homolog, negatively regulates microRNA transcription

Project description:We performed ChIP-seq analysis of LIN-42 from extracts obtained from staged late L4 hermaphrodites. We found 413 high confidence peaks that are predominately found at promoter regions and transcription start sites of mRNA and microRNA genes. ChIP assays using a functional GFP tagged transgene of LIN-42 using an anti-GFP antibody, as well as using an antibody raised agans the endogenous LIN-42 protein.

2014-06-03 | E-GEOD-58171 | biostudies-arrayexpress

Effect of lin-28 and lin-46 on miRNA profile in late L1 stage Caenorhabditis elegans

Project description:Comparison of miRNA profiles of wildtype and lin-28(n719); lin-46(ma164) Caenorhabditis elegans nematodes at the L1 stage Two genotypes, wildtype vs. mutant. Biological replicates: 3 wild type, 3 mutant, independently grown and harvested. One replicate per slide.

2012-02-09 | E-GEOD-35634 | biostudies-arrayexpress

LIN-42, the Caenorhabditis elegans PERIOD homolog, negatively regulates microRNA transcription

2014-06-03 | GSE58171 | GEO