Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Boechera divaricarpa

ABSTRACT: Boechera divaricarpa overview

PROVIDER: PRJNA251443 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR1587838_1.fastq.gz	Fastqsanger.gz
	SRR1587838_2.fastq.gz	Fastqsanger.gz
	SRR1587839_1.fastq.gz	Fastqsanger.gz
	SRR1587839_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Boechera divaricarpa isolate:M4B

Project description:Chromosome-level diploid genome assembly of apomictic Boechera hybrid M4B

| PRJNA774175 | ENA

Boechera divaricarpa cultivar:ES517

Project description:Hi-C assisted chromosome-level genome assembly of apomictic Boechera divaricarpa ES517

| PRJNA765627 | ENA

Boechera divaricarpa strain:ES 517

Project description:Boechera divaricarpa strain:ES 517 Genome sequencing

| PRJNA209546 | ENA

CNV in sexual and apomictic Boechera

Project description:The absence of meiosis and sex are expected to lead to mutation accumulation in asexual (apomictic) plants. We have performed a double-validated analysis of copy number variation (CNV) on 10 biological replicates each of diploid sexual and diploid apomictic Boechera using a high-density (>700K) custom microarray, in order to compare mutation accumulation in the form of CNV between the transcribing regions of their genomes. The Boechera genome demonstrated higher levels of depleted compared to enriched CNV, irrespective of reproductive mode. Genome-wide patterns of CNV revealed four divergent lineages, three of which were characterized by both sexual and apomictic genotypes. Hence genome-wide CNV is reflective of at least 3 independent origins (i.e. expression) of apomixis from different sexual genetic backgrounds. CNV distributions for different families of transposable elements (TEs) were lineage specific, and a trend for enrichment in LINE/L1 and LTR/Copia elements in lineage 3 apomicts is consistent with sex and meiosis being mechanisms for purging genomic parasites. We hypothesize that significant overrepresentation of specific gene ontology classes (e.g. pollen-pistil interaction) in apomicts implies that gene enrichment could be an adaptive mechanism for genome stability in diploid apomicts by providing a polyploid-like system for buffering the effects of deleterious mutations.

2014-04-07 | GSE51596 | GEO

Boechera divaricarpa strain:ES 517

Project description:Boechera divaricarpa strain:ES 517 Transcriptome or Gene expression

| PRJNA250814 | ENA

Expression analysis of uninfected Boechera stricta and infected Boechera stricta with Puccinia monoica forming pseudoflowers using an Arabidopsis thaliana gene expression 4x72K array design from NimbleGen.

2013-09-01 | GSE41165 | GEO

Different transcript patterns in response to specialist and generalist herbivores in the wild Arabidopsis relative Boechera divaricarpa.

Project description:BACKGROUND: Plants defend themselves against herbivorous insects, utilizing both constitutive and inducible defenses. Induced defenses are controlled by several phytohormone-mediated signaling pathways. Here, we analyze transcriptional changes in the North American Arabidopsis relative Boechera divaricarpa in response to larval herbivory by the crucifer specialist lepidopteran Plutella xylostella (diamondback moth) and by the generalist lepidopteran Trichoplusia ni (cabbage semilooper), and compare them to wounding and exogenous phytohormone application. METHODOLOGY/PRINCIPAL FINDINGS: We use a custom macroarray constructed from B. divaricarpa herbivory-regulated cDNAs identified by suppression subtractive hybridization and from known stress-responsive A. thaliana genes for transcript profiling after insect herbivory, wounding and in response to jasmonate, salicylate and ethylene. In addition, we introduce path analysis as a novel approach to analyze transcript profiles. Path analyses reveal that transcriptional responses to the crucifer specialist P. xylostella are primarily determined by direct effects of the ethylene and salicylate pathways, whereas responses to the generalist T. ni are influenced by the ethylene and jasmonate pathways. Wound-induced transcriptional changes are influenced by all three pathways, with jasmonate having the strongest effect. CONCLUSIONS/SIGNIFICANCE: Our results show that insect herbivory is distinct from simple mechanical plant damage, and that different lepidopteran herbivores elicit different transcriptional responses.

| S-EPMC2031921 | biostudies-literature

Project description:The expression of 30362 plant genes from uninfected flowers of Boechera stricta, uninfected steam and leaves of B. stricta and infected B. stricta with Puccinia monoica forming pseudoflowers. We hybridized cDNA from each sample to an Arabidopsis thaliana gene expression 4x72K format NimbleGen array (ATH6_60mer_expr). We used a eukaryotic gene expression array design No.5048 from NimbleGen (Cat No. A4511001-00-01). Each 5048 array measures the expression level of 30,362 target genes from Arabidopsis thaliana in a 4-plex format 4x72K with with 72,000 probes per array, a total of two probes per target gene, and 60-mer probe length. Total RNA samples recovered from infected leaves of Boechera stricta with Puccinia monoica (pseudoflowers) and uninfected stem and leaves of B. stricta. Experiments included three/two biological repllicates from each sample. We carried out total RNA extractions for all samples using RNAesy Plant Mini Kit (Qiagen, Cat No. 74904). cDNA synthesis was performed by NimbleGen.

2013-09-01 | E-GEOD-41165 | biostudies-arrayexpress

Expression profiling of immature ovule and pistil samples of sexual and apomictic Boechera (Brassicaceae)

Project description:Sexual reproduction (meiosis and syngamy) is the major form of reproduction in diploid Boechera species, but most species hybrids reproduce by apomixis (unreduced gametophyte formation followed by parthenogenesis of the unreduced egg). In this study, we used Arabidopsis microarrays to detail global programs of gene expression underlying sexual and apomictic modes of reproduction.

2020-11-30 | GSE156684 | GEO

Boechera holboellii

Project description:Boechera holboellii overview

| PRJNA61541 | ENA