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Sus scrofa

ABSTRACT: Differential microRNA expression and microRNA editing upon HP-PRRSV infection in vivo across different pig breeds

PROVIDER: PRJNA252609 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1383530.fastq.gz	Fastqsanger.gz
	SRR1383531.fastq.gz	Fastqsanger.gz
	SRR1383532.fastq.gz	Fastqsanger.gz
	SRR1383533.fastq.gz	Fastqsanger.gz
	SRR1383526.fastq.gz	Fastqsanger.gz

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Sus scrofa

Project description:Genetic variation contribute to different susceptibility to HP-PRRSV infection across pig breeds

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Differential microRNA expression and microRNA editing upon HP-PRRSV infection in vivo across different pig breeds

Project description:Purpose: To characterize the differential microRNA expression profiles and microRNA editing upon PRRSV infection, using NGS techonology, we sequenced small RNAs of the lungs of Tongcheng and Landrace pigs before (0 dpi) and after (3, 5, 7 dpi) infection with high-pathogenic PRRSV (HP-PRRSV). Methods: The miRNA expression profiles of the lungs of Tongcheng and Landrace pigs before (0 dpi) and after (3, 5, 7 dpi) HP-PRRSV infection were produced by using solexa platform. The raw reads with low qualities were filtered and the clean high quality reads were mapped to Ensembl Sus reference genome 10.2.71 using BWA. The unique mapped reads were retained for microRNA expression analysis. The raw reads counts of each microRNA were calculated by perl scripts and the differentially expressed microRNA (Fold change >2; FDR <0.05) were called using edgeR. The microRNA editing was identified using the methods described by Alon, S. and E. Eisenberg. Methods Mol Biol, 2013. Further analysis of microRNA editing was performed with perl scripts.

2014-10-10 | GSE58436 | GEO

Genetic variation contribute to different susceptibility to HP-PRRSV infection across pig breeds

Project description:Purpose: To characterize the differential mRNA expression profiles of lung tissues upon PRRSV infection in different pig breeds, using NGS techonology, we sequenced mRNAs of the lungs of Tongcheng and Landrace pigs before (0 dpi) and after (3, 5, 7 dpi) infection with high-pathogenic PRRSV (HP-PRRSV). Methods: The mRNA expression profiles of the lungs of Tongcheng and Landrace pigs before (0 dpi) and after (3, 5, 7 dpi) HP-PRRSV infection were produced by using solexa platform. The raw reads with low qualities were filtered and the clean high quality reads were mapped to Ensembl Sus reference genome 10.2.71 using BOWTIE2. The unique mapped reads were retained for mRNA expression analysis. The raw reads counts of each mRNA were calculated by HTseq and the differentially expressed mRNA (Fold change >2; FDR <0.05) were called using DEGseq.

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Sus scrofa skeletal muscle degradation and deposition

Project description:a 4D-label free detection method using LC-MS/MS was employed to analyze the skeletal muscle samples from different pig breeds

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Project description:Pulmonary alveolar macrophages (PAMs) of Tongcheng piglets response to HP-PRRSV infection in vivo

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Project description:CGH analyses for the Chinese indigenous pig breeds and commercial pig breeds

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Project description:SNP genotyping analyses for the Chinese indigenous pig breeds and commercial pig breeds

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Project description:CGH analyses for the Chinese indigenous pig breeds and commercial pig breeds [Agilent]

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DH06 Adrenal ACTH Sus scrofa

Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig

2008-01-07 | GSE8377 | GEO

DH06 Adrenal ACTH Sus scrofa

Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 Âµg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80Â°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples

2010-06-29 | E-GEOD-8377 | biostudies-arrayexpress

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