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Spatiotemporal regulation of GLI target genes in the mammalian limb bud

ABSTRACT: Spatiotemporal regulation of GLI target genes in the mammalian limb bud

PROVIDER: PRJNA253126 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1425520_1.fastq.gz	Fastqsanger.gz
	SRR1425520_2.fastq.gz	Fastqsanger.gz
	SRR1425940_1.fastq.gz	Fastqsanger.gz
	SRR1425940_2.fastq.gz	Fastqsanger.gz
	SRR1425521_1.fastq.gz	Fastqsanger.gz

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Early limb bud transcriptome analysis between anterior and posterior domain

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Project description:Analysis of mouse limb bud (E10.5) lacking the Bhlha9 gene. Bhlha9 knockout mouse shows syndactyly and poliosis in the limb. This microarray results provides insight into the molecular mechanisms underlying Bhlha9 function in the limb development DNA microarray analysis was performed using Affymetrix mouse genome 430 2.0 array. RNA samples were obtained from the whole limb bud of the E10.5 wild-type and Bhlha9 knockout embryos described above. Total RNA (200 ng) was reverse-transcribed and biotinylated using the GeneChip 3â² IVT Express Kit (Affymetrix). The microarray data were summarized using the MAS 5.0 method.

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