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MiRNAs 182 and 183 are necessary to maintain adult cone photoreceptor outer segments and visual function

ABSTRACT: MiRNAs 182 and 183 are necessary to maintain adult cone photoreceptor outer segments and visual function

PROVIDER: PRJNA253145 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1427140.fastq.gz	Fastqsanger.gz
	SRR1427150.fastq.gz	Fastqsanger.gz
	SRR1427160.fastq.gz	Fastqsanger.gz
	SRR1427141.fastq.gz	Fastqsanger.gz
	SRR1427151.fastq.gz	Fastqsanger.gz

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MicroRNA expression profile in mouse cone photoreceptors at P60 [miRNA-seq]

Project description:The outer segments of cones serve as light detectors for daylight color vision, and their dysfunction leads to human blindness conditions. We show that the cone-specific disruption of DiGeorge Syndrome Critical Region Gene 8 (DGCR8) in adult mice led to the loss of miRNAs and the loss of outer segments, resulting in photoreceptors with significantly reduced light responses. Using next-generation sequencing of RNA from isolated wild type P60 cones, we determine the most highly expressed miRNAs as candidates for controlling outer segment maintenance. The expression pattern of miRNAs was highly uneven, with a single miRNA, miR-182, representing 64% of all miRNA expressed in cones. Re-expression of miR-182 and miR-183 (third most abundant miRNA) prevented outer segment loss. These miRNAs were also necessary and sufficient for the formation of inner segments, connecting cilia and short outer segments, as well as light responses in stem-cell-derived retinal cultures. Our results show that miR-182- and miR-183-regulated pathways are necessary for cone outer segment maintenance in vivo and functional outer segment formation in vitro.

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MicroRNA expression profile in mouse cone photoreceptors at P60 [miRNA-seq]

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MiRNAs 182 and 183 are necessary to maintain adult cone photoreceptor outer segments and visual function

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-06-20 | GSE58501 | GEO

MiRNAs 182 and 183 are necessary to maintain adult cone photoreceptor outer segments and visual function

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-06-20 | E-GEOD-58501 | biostudies-arrayexpress

The microRNA cluster miR-183/96/182 contributes to long-term memory in a protein phosphatase 1 dependent manner

Project description:Memory formation is a complex cognitive function regulated by coordinated synaptic and nuclear processes in neurons. In mammals, it is controlled by multiple molecular activators and suppressors, including the key signaling regulator protein phosphatase 1 (PP1). Here, we show that memory control by PP1 involves the miR-183/96/182 cluster, which is selectively regulated during memory formation. Inhibiting nuclear PP1 in mice brain or training in object recognition task similarly increases miR-183/96/182 expression in the hippocampus. Mimicking this increase by overexpressing miR-183/96/182 enhances object memory, while suppressing endogenous level of the cluster reduces it. This effect involves the modulation of many plasticity-related genes, and we identified HDAC9 as one of the functional targets. Further, PP1 controls miR-183/96/182 in a transcription-independent manner influencing processing of their precursors. These findings provide novel evidence for the role of miRNAs in memory formation and suggest the implication of PP1 in miRNAs processing in the adult brain.

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