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Homo sapiens

ABSTRACT: RepliSeq of HT-1080 cell line

PROVIDER: PRJNA253850 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1482430_1.fastq.gz	Fastqsanger.gz
	SRR1482430_2.fastq.gz	Fastqsanger.gz
	SRR1482431_1.fastq.gz	Fastqsanger.gz
	SRR1482431_2.fastq.gz	Fastqsanger.gz
	SRR1482426_1.fastq.gz	Fastqsanger.gz

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RepliSeq of HT-1080 cell line

2014-07-31 | GSE58907 | GEO

Development of gene expression signatures for subconfluent and overconfluent HT-1080 fibrosarcoma cell line

Project description:We investigated the changes in gene expression profile between subconfluent (80%) and overconfluent HT-1080 fibrosarcoma cell line. We have employed whole genome microarray expression profiling as a discovery platform to identify genes expression profiles in HT-1080 cells. Total RNAs from subconfluent (HT-1080_80%) and overconfluent (HT-1080_overconfluent) HT-1080 cells were harvested and subjected to the microarray analysis.

2015-06-04 | E-GEOD-69534 | biostudies-arrayexpress

RepliSeq of HT-1080 cell line

Project description:Analysis of replication timing by RepliSeq of HT-1080 Human Connective Tissue Fibrosarcoma Cells. HT-1080 subclone +4 cell line as described in “Human gene targeting favors insertions over deletions.” Russell, D.W., and Hirata, R.K. (2008). Hum Gene Ther 19, 907-914. RepliSeq of HT-1080 cell line was used to determine the impact of DNA replication on targeted adeno-associated virus sites by mapping replication forks, which revealed a consistent preference for recombination at target loci transcribed towards an incoming fork.

2014-07-31 | E-GEOD-58907 | biostudies-arrayexpress

Development of gene expression signatures for subconfluent and overconfluent HT-1080 fibrosarcoma cell line

Project description:We investigated the changes in gene expression profile between subconfluent (80%) and overconfluent HT-1080 fibrosarcoma cell line.

2015-06-04 | GSE69534 | GEO

Homo sapiens

Project description:Development of gene expression signatures for subconfluent and overconfluent HT-1080 fibrosarcoma cell line

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Proteomic analysis of HT-1080 cells overexpressing protein ARGLU1

Project description:A proteomic comparison between normal HT-1080 cells and HT-1080 cells that have been stably transfected to overexpress protein arginie and glutamate rich 1 (ARGLU1).

2024-05-13 | PXD046329 | Pride

Homo sapiens

Project description:Expression Data for HT-1080 cells exposed to ETP, QUE and MMS

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RAMPAGE from HT1080 (ENCSR348DYP)

Project description:The libraries contained in this experiment come from independent growths of cell line HT-1080, a connective tissue fibrosarcoma from a 35 year old male. They are stranded PE101 Illumina Hi-Seq RAMPAGE libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2016-11-08 | GSE88210 | GEO

total RNA-seq from HT1080 (ENCSR535VTR)

Project description:The libraries contained in this experiment come from independent growths of cell line HT-1080, a connective tissue fibrosarcoma from a 35 year old male. They are stranded PE101 Illumina Hi-Seq libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2016-03-18 | GSE78653 | GEO

Crystal structure of Homo sapiens kynureninase.

Project description:Kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5'-phosphate (PLP) dependent enzymes known as the aspartate aminotransferase superfamily or alpha-family. The Homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. The human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacterial expression system using Ni metal ion affinity chromatography. Kinetic characterization of the recombinant enzyme reveals classic Michaelis-Menten behavior, with a Km of 28.3 +/- 1.9 microM and a specific activity of 1.75 micromol min-1 mg-1 for 3-hydroxy-dl-kynurenine. Crystals of recombinant kynureninase that diffracted to 2.0 A were obtained, and the atomic structure of the PLP-bound holoenzyme was determined by molecular replacement using the Pseudomonas fluorescens kynureninase structure (PDB entry 1qz9) as the phasing model. A structural superposition with the P. fluorescens kynureninase revealed that these two structures resemble the "open" and "closed" conformations of aspartate aminotransferase. The comparison illustrates the dynamic nature of these proteins' small domains and reveals a role for Arg-434 similar to its role in other AAT alpha-family members. Docking of 3-hydroxy-l-kynurenine into the human kynureninase active site suggests that Asn-333 and His-102 are involved in substrate binding and molecular discrimination between inducible and constitutive kynureninase substrates.

| S-EPMC2531291 | biostudies-literature

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